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فهرست مطالب: Preface . . . . vContributors. . . . . xi 1 Archaea: A Goldmine for Molecular Biologists and Evolutionists . . . 1 PART I GENETIC MANIPULATION OF ARCHAEA 2 Progress and Challenges in Archaeal Genetic Manipulation . . . 25 3 Genetic Manipulation of Haloferax Species. . . 33 4 CRISPR Interference as a Tool to Repress Gene Expression in Haloferax volcanii . . 57 5 Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis. . . 87 6 Genetic Methods and Construction of Chromosomal Mutations in Methanogenic Archaea . . . 105 7 An Interdomain Conjugation Protocol for Plasmid-DNA Transfer into Methanothermobacter thermautotrophicus ΔH . 119 8 Methods for Markerless Gene Deletion and Plasmid-Based Expression in Sulfolobus acidocaldarius. . 135 9 A Rapid Targeted Gene Inactivation Approach in Sulfolobus islandicus. . . . 145 10 Transposon Insertion Mutagenesis in Hyperthermophilic Crenarchaeon Sulfolobus islandicus . . . 163 11 Reprogramming CRISPR-Mediated RNA Interference for Silencing of Essential Genes in Sulfolobales . . . . . . 177 PART II MOLECULAR BIOLOGY OF ARCHAEA 12 Progress and Challenges in Archaeal Molecular Biology . 205 13 ChIP-Seq Occupancy Mapping of the Archaeal Transcription Machinery . 209 14 Conversion of Ribosome-Protected mRNA to DNA and Deep Sequencing for Ribosome Profiling in Haloferax volcanii . . . . . 223 15 Small RNA-Sequencing Library Preparation for the Halophilic Archaeon Haloferax volcanii . . . . . 243 16 Quantitative Mass Spectrometry by SILAC in Haloferax volcanii . . . 255 17 Proteome Turnover Analysis in Haloferax volcanii by a Heavy Isotope Multilabeling Approach. . . . . 267 18 Proteomic Sample Preparation and Data Analysis in Line with the Archaeal Proteome Project . . . . 287 19 In Vivo Protein Cross-Linking and Coimmunoprecipitation in Haloferax volcanii . . 301 20 Proteolytic Activity Assays in Haloarchaea. . . . 319 21 Carotenoids from Haloarchaea: Extraction, Fractionation, and Characterization . . 331 22 Metal nanoparticles Biosynthesis Using the Halophilic Archaeon Haloferax volcanii . . . . 345 23 Simplified Enzymatic Synthesis of 2-Keto-3-Deoxy-D-Gluconate from D-Gluconate Using the Gluconate Dehydratase from Thermoproteus tenax. . . 351 PART III CELLULAR BIOLOGY OF ARCHAEA 24 Progress and Challenges in Archaeal Cell Biology. . 365 25 Methods to Analyze Motility in Eury- and Crenarchaea . 373 26 Immersed Liquid Biofilm and Honeycomb Pattern Formations in Haloferax volcanii . . 387 27 Cost-Effective and Versatile Analysis of Archaeal Surface Adhesion Under Shaking and Standing Conditions . . . . 397 28 Cell Adhesion and Biofilm Formation Analysis . . . . 407 29 BrdU Incorporation and Labeling of Nascent DNA to Investigate Archaeal Replication Using Super-Resolution Imaging . . 419 30 Isolation, Purification, and Characterization of Membrane Vesicles from Haloarchaea . . . . . 435 31 Archaeal Viruses: Production of Virus Particles and Vesicle-like Viruses and Purification Using Asymmetrical Flow Field-Flow Fractionation . . . . . 449 PART IV ENVIRONMENTAL BIOLOGY 32 Progress and Challenges in Studying the Ecophysiology of Archaea . 469 33 Reconstruction of Archaeal Genomes from Short-Read Metagenomes . . . 487 PART V OUTREACH PROTOCOL 34 Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii. 531 Index . . . . . 547 مشخصات فایل |
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| عنوان (Title): | Archaea_ Methods and Protocols |
| نام فایل (File name): | 930-www.GeneProtocols.ir-Archaea_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): | آرکی ها - روشها و پروتکل ها |
| ایجاد کننده: | Sébastien Ferreira-Cerca |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 107162444X, 9781071624449 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 15.6 مگابایت |
| تعداد صفحات (Book length in pages): | 542 |
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2022,
آرکی ها,
انگلیسی,
باکتری,
باکتری شناسی,
میکروارگانیسم,
میکروبیولوژی,
پروتکل آزمایشگاهی,
پروتکل میکروبی,
