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فهرست مطالب: Preface . . . . . . . . . . . . . . vContributors. . . . . . . . . . . . . . . ix PART I PRODUCTION AND DESIGN 1 Antibiotics: Precious Goods in Changing Times . . . . . . . . 3 Peter Sass 2 A Whole-Cell Assay for Detection of Antibacterial Activity in Actinomycete Culture Supernatants. . . . . . . 27 Anika Ru¨tten, Wolfgang Wohlleben, Lena Mitousis, and Ewa Maria Musiol-Kroll 3 Sampling of Human Microbiomes to Screen for Antibiotic-Producing Commensals. . . . . . . . . . 39 Benjamin Torres Salazar, Anna Lange, Laura Camus, and Simon Heilbronner 4 Production of Antimicrobial Compounds by Homologous and Heterologous Expression . . . . . . . . . 55 I. Dewa M. Kresna, Zerlina G. Wuisan, and Till F. Schaberle € 5 Isolation and Purification of Natural Products from Microbial Cultures. . . 75 Thomas Schafhauser and Andreas Kulik 6 Structure Elucidation of Antibiotics . . . . . . . . . 97 Julia Moschny, Georgios Daletos, Peter Proksch, and Chambers C. Hughes 7 Computer-Aided Drug Design: An Update . . . . . . . 123 Wenbo Yu, David J. Weber, and Alexander D. MacKerell Jr 8 Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents. . . . . . . . . . 153 Alexander Zipperer, Jasmin Scheurer, and Dorothee Kretschmer PART II MODE OF ACTION AND RESISTANCE 9 Microscopy-Based Multiwell Assay to Characterize Disturbed Bacterial Morphogenesis Upon Antibiotic Action . . . . . . . 171 Cruz L. Matos de Opitz and Peter Sass 10 Expansion Microscopy of Bacillus subtilis . . . . . . . . . 191 Viola Middelhauve, Jan Peter Siebrasse, and Ulrich Kubitscheck 11 Tracking Global and Local Changes in Membrane Fluidity Through Fluorescence Spectroscopy and Microscopy . . . . . . . . 203 Madeleine Humphrey, Ireny Abdelmesseh Nekhala, Kathi Scheinpflug, Oxana Krylova, Ann-Britt Schafer, Jessica A. Buttress, € Michaela Wenzel, and Henrik Strahl 12 Quantitative Analysis of Microscopy Data to Evaluate Bacterial Responses to Antibiotic Treatment. . . . . . . . . . 231 Dominik Brajtenbach, Jan-Samuel Puls, Cruz L. Matos de Opitz, Peter Sass, Ulrich Kubitscheck, and Fabian Grein 13 Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds . . . . . . 259 Carolin Martina Kobras, Sali May Morris, Thorsten Mascher, and Susanne Gebhard 14 Determination of Bacterial Membrane Impairment by Antimicrobial Agents . . . . . . . . . 271 Miriam Fuerst-Wilmes and Hans-Georg Sahl 15 A Colorimetric Assay to Identify and Characterize Bacterial Primase Inhibitors . . . . . . . . . 283 Allan H. Pang and Oleg V. Tsodikov 16 Cell-Based Fluorescent Screen Amenable to HTS to Identify Inhibitors of Bacterial Translation Initiation. . . . . . . 303 Matteo Raneri, Emilio Alvarez-Ruiz, Danuta Mossakovska, and Federica Briani 17 Bacterial Two Component Systems: Overexpression and Purification: In Vitro and In Vivo Inhibitor Screens . . . . . . 313 Alina Dietrich, Mike Gajdiss, Michael Tu¨rck, Ian Monk, and Gabriele Bierbaum 18 Sample Preparation for Mass Spectrometry-Based Absolute Quantification of Bacterial Proteins in Antibiotic Stress Research . . . 335 Sandra Maaß, Minia Antelo-Varela, Florian Bonn, and Do ¨rte Becher 19 Elemental Analysis for the Characterization of Antimicrobial Effects . . . . . 349 Christoph H. R. Senges and Julia E. Bandow 20 Label-Free Quantitation of Ribosomal Proteins from Bacillus subtilis for Antibiotic Research . . . . . . . . . . 363 Sina Schakermann, Pascal Dietze, and Julia E. Bandow € 21 Functional Metagenomics to Study Antibiotic Resistance . . . . . 379 Bejan Mahmud, Manish Boolchandani, Sanket Patel, and Gautam Dantas Index . . . . . . . . . . . . . . . 403 مشخصات فایل |
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| عنوان (Title): | Antibiotics_ Methods and Protocols |
| نام فایل (File name): | 992-www.GeneProtocols.ir-Antibiotics_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
آنتی بیوتیک ها- روشها و پروتکل ها
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| ایجاد کننده: | Peter Sass |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071628546 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 13.3 مگابایت |
| تعداد صفحات (Book length in pages): | 407 |
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