-
قابل دانلود از شنبه, ۶ خرداد ۱۴۰۲
|
فهرست مطالب: Preface .......... . . . . . vContributors.......... . ix 1 A DNA Pull-Down Assay with Diversity Forms of Competitor for Detecting or Evaluating Protein–DNA Interactions .... 1 Hongyan Sui and Tomozumi Imamichi 2 Enhanced Yeast One-Hybrid Assays to Study Protein–DNA Interactions ....... . . . . 11 Anna Berenson and Juan Ignacio Fuxman Bass 3 Chromatin Immunoprecipitation on Fixed Tissues and Cell Lines .. . . . . 21 Dimitrius Pramio and Deborah Schechtman 4 The ChIP-Exo Method to Identify Genomic Locations of DNA-Binding Proteins at Near Single Base-Pair Resolution ..... . . 33 Ssu-Yu Yeh and Ho Sung Rhee 5 ChIP-Seq Protocol for In Vitro Cell Differentiation Systems ... . . 49 Jacqueline Copeland, Fjodor Merkuri, and Marcos Simoes-Costa 6 Profiling Accessible Chromatin and Nucleosomes in the Mammalian Genome ....... . . . 59 Hee-Woong Lim and Makiko Iwafuchi 7 Dissecting Locus-Specific Chromatin Interactions by CRISPR CAPTURE........ 69 Giovanni A. Botten, Michael Lee Jr, and Jian Xu 8 CUT & RUN to Profile Chromatin-Bound Proteins in Primary Mouse Neural Progenitor Cells ....... 99 Yurika Matsui and Jamy C. Peng 9 Easy Hi-C: A Low-Input Method for Capturing Genome Organization .113 Leina Lu and Fulai Jin 10 Single Molecule Imaging of DNA–Protein Interactions Using DNA Curtains........ . . 127 J. Brooks Crickard 11 Quantifying the Binding and Target-Search Kinetics of Transcriptional Regulatory Factors by Live-Cell Single-Molecule Tracking... . . . . 141 Steven Ingersoll, Kyle Brown, Brian Ma, and Xiaojun Ren 12 Preparation of Recombinant Histones and Widom 601 DNA for Reconstitution of Nucleosome Core Particles .... . . . . . 163 Emeleeta A. Paintsil and Emma A. Morrison 13 Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA ......177 Erin F. Hammonds and Emma A. Morrison vii viii Contents 14 Rapid Single-Pot Assembly of Modular Chromatin Proteins for Epigenetic Engineering....... . . . . 191 Karmella A. Haynes and J. Harrison Priode 15 Mapping Transcription Regulation with Run-on and Sequencing Data Using the Web-Based tfTarget Gateway..... . . 215 Nating Wang, Zhong Wang, Charles G. Danko, and Tinyi Chu 16 In Embryo Gene Reporter Assays for Evaluation of Cis-Regulatory Regions ....... . . . . 227 Carolina Purcell Goes, Tatiane Y. Kanno, and C. Y. Irene Yan 17 In Situ Hybridization as a Method to Examine Gene Regulatory Activity In Vivo......... 241 Rhea R. Datta and Pinar Onal 18 Investigating Tissue Regeneration Using the DUAL Control Genetic Ablation System ........255 R. E. Harris 19 Fishing for Developmental Regulatory Regions: Zebrafish Tissue-Specific ATAC-seq....... . . . . . 271 Rekha M. Dhillon-Richardson, Alexandra K. Haugan, and Megan L. Martik Index ..........283 مشخصات فایل |
|
| عنوان (Title): | DNA-Protein Interactions_ Methods and Protocols |
| نام فایل (File name): | 990-www.GeneProtocols.ir-DNA-Protein Interactions_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
اثرات متقابل دی ان ای و پروتئین - روشها و پروتکل ها
|
| ایجاد کننده: | Marcos Simoes-Costa |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071628461 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 14 مگابایت |
| تعداد صفحات (Book length in pages): | 288 |
پس از پرداخت، دانلود فایل آغاز می شود
درباره درگاه پرداخت نکست پی بیشتر بدانید
تمامی درگاه های پرداخت ژنـ پروتکل توسط شرکت دانش بنیان نکست پی پشتیبانی می شود. نکست پی دارای مجوز رسمی پرداختیاری به شماره 1971/ص/98 ، از شرکت شاپرک و بانک مرکزی جمهوری اسلامی ایران و دارای نماد اعتماد در حوزه (متمرکزکنندگان پرداخت) از مرکز توسعه تجارت الکترونیکی وزارت صنعت معدن و تجارت است.
بیوکمیکال 