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فهرست مطالب: Preface .......... . . . . . vContributors.......... . ix 1 Inclusion Bodies: Status Quo and Perspectives..... . 1 Julian Kopp and Oliver Spadiut PART I UPSTREAM PROCESSING 2 Thermoinducible E. coli for Recombinant Protein Production in Inclusion Bodies........ . . . . 17 Norma A. Valdez-Cruz and Mauricio A. Trujillo-Rolda´ n 3 High-Throughput Expression of Inclusion Bodies on an Automated Platform ....... . . . . 31 Annina Kemmer, Linda Cai, M. Nicolas Cruz Bournazou, and Peter Neubauer 4 Design, Production, and Characterization of Catalytically Active Inclusion Bodies ........ 49 Gizem O¨ lc¸u¨cu¨, Karl-Erich Jaeger, and Ulrich Krauss 5 Bidirectional Promoter Libraries Enable the Balanced Co-expression of Two Target Genes in E. coli ..... . 75 Julia Jodlbauer, Lukas Rieder, Anton Glieder, and Birgit Wiltschi 6 Inclusion Body Production in Fed-Batch and Continuous Cultivation ......... . . . . 87 Stefan Kittler 7 Avoiding the All-or-None Response in Gene Expression During E. coli Continuous Cultivation Based on the On-Line Monitoring of Cell Phenotypic Switching Dynamics .... . . 103 Frank Delvigne, Lucas Henrion, Vincent Vandenbroucke, and Juan Andres Martinez 8 Optimization of Inclusion Body Formation and Purification in Multi-well Plates........ . . . . 121 Sarah Kamel, Julia Schollmeyer, Anke Kurreck, and Peter Neubauer PART II DOWNSTREAM PROCESSING 9 High Pressure Homogenization for Inclusion Body Isolation ... . 141 Julian Ebner, Viktor Sedlmayr, and Robert Klausser 10 Development of Solubilization and Refolding Buffers .... . 155 Mohammad Roufarshbaf and Vajihe Akbari 11 Unit Operation-Spanning Investigation of the Redox System ... . 165 Julian Ebner, Diana Humer, and Viktor Sedlmayr 12 Using High Pressure and Alkaline pH for Refolding .... . . 177 Ligia Morganti and Rosa Maria Chura-Chambi 13 Intensification of Inclusion Body Processing via Temperature-Based Refolding ....... . 189 Ru Shen Wong, Nurul Nadia Mohamad Alias, Eugene Boon Beng Ong, and Mervyn W. O. Liew 14 Refolding of Proteins Expressed as Inclusion Bodies in E. coli ... . 201 Rashmi Sharma, Anupa Anupa, and Anurag S. Rathore 15 Infrared Spectroscopy for Structure Analysis of Protein Inclusion Bodies ........209 Andreas Schwaighofer and Bernhard Lendl PART III SUCCESS STORIES 16 The Purification of Heme Peroxidases from Escherichia coli Inclusion Bodies: A Success Story Shown by the Example of Horseradish Peroxidase ....... . . . . 227 Diana Humer and Julian Ebner 17 Production of α-Synuclein Fibrillar-Specific scFv from Inclusion Bodies ........ . 239 Vijay Gupta, Issam Hmila, Nishant N. Vaikath, Indulekha P. Sudhakaran, and Omar M. A. El-Agnaf 18 Method for Inclusion Bodies Production via E. coli Host System: rGCSF as Model Biotherapeutic Protein .... 249 Abhilasha K. Rani, Venkateswara R. Naira, and Anurag S. Rathore 19 Antimicrobial Applications of Inclusion Bodies ..... 257 Ramon Roca-Pinilla, Anna Arı´s, and Elena Garcia-Fruitos Index ..........271 مشخصات فایل |
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| عنوان (Title): | Inclusion Bodies_ Methods and Protocols |
| نام فایل (File name): | 1002-www.GeneProtocols.ir-Inclusion Bodies_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
اجسام اینکلوژن - روش ها و پروتکل ها
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| ایجاد کننده: | Julian Kopp, Oliver Spadiut |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071629291 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 7.95 مگابایت |
| تعداد صفحات (Book length in pages): | 275 |
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