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فهرست مطالب: Foreword................. . . . vPreface to the Series.............. . . . vii Preface................ . . . . . ix Contributors................. xv PART I IN VITRO BIOSAFETY ASSESSMENT OF PROBIOTICS 1 Determination of Biogenic Amine Production....... . . . . . 3 2 Determination of Gelatinases, Glycosidases, and Enolase Production.. . . 17 3 Determination of β-Glucuronidase Production...... . . . . . 29 4 Determination of Nitroreductase Production....... . . . . . . 33 5 Determination of Azoreductase Production........ 39 6 Determination of Hemolytic Activity........ . . . . . . 43 7 Determination of DNAse Activity........... 47 8 Determination of Bile Salts Deconjugation......... 53 9 Determination of D-Lactic Acid Production........61 10 Determination of Antibiotic Resistance........ . . . . 71 11 Determination of Antibiotic Resistance Gene Transfer..... . . . . . . 85 12 Determination of Toxin Production......... . . . . . . 95 13 Detection of Toxin Genes by PCR Based Methods....... . 107 14 Evaluation of Pathogenicity Potential by Phenotypic and Genotypic Methodologies.............. . 123 15 Determination of Toxicity Through Cytotoxicity Assays.... . . . . . 137 16 In Vitro Evaluation of the Nitric Oxide Pathway....... . . . 149 17 Assessment of Capsule Formation........... 157 18 Assessment of Platelet Aggregation.........165 19 Assessment of Fibrinogen and Fibronectin Binding Activity.... . . 171 20 Assessment of Probiotics Adhesion to Mammalian Cells.... . . . . . 179 21 Assessment of Mutagenicity.......... . . . . . . 185 22 Assessment of Induction and Destruction of Thrombi..... . . . . . . 201 23 Assessment of Degradation of Mucin......... . . . . . 211 PART II IN VIVO BIOSAFETY ASSESSMENT OF PROBIOTICS: MONITORING IN VIVO TOXICITY OF PROBIOTICS 24 Evaluation of General Health Status of the Animals During the In-Life Phase............ 225 25 Assessment of Bacterial Translocation Through Blood Cultures.. . . . . . . 237 26 Determination of Splenic Weight Index and Weight-to-Length Ratio.. . 243 27 Determination of Total Liver Glutathione and Plasma Malondialdehyde Concentrations.......... . 253 28 Determination of Serum Lactate and Fecal Calprotectin for Assessing the Intestinal Inflammation.......... . . . . . . 267 PART III IN VIVO BIOSAFETY ASSESSMENT OF PROBIOTICS: MEASURING INFECTIVITY IN ANIMAL MODELS 29 In Vivo Evaluation of Adhesion Properties of Probiotics..... . . . . 279 30 Determination of Streptomyces Probiotics Oral Administration in Broiler Chicken............ . . . . . . 287 31 Determination of Infectivity of Probiotics Using Animal Model.. . . . . . . 299 32 Determination of Infectivity Using Immunosuppressed Hosts....309 PART IV IN VIVO BIOSAFETY ASSESSMENT OF PROBIOTICS: MEASURING REPRODUCTIVE AND DEVELOPMENTAL TOXICITY IN ANIMAL MODELS 33 Assessment of Reproductive Toxicity........ . . . . . . 329 34 Assessment of Developmental Toxicity in Zebrafish Model..... . . 339 PART V IN VIVO BIOSAFETY ASSESSMENT OF PROBIOTICS: MEASURING IMMUNOLOGICAL PARAMETERS IN ANIMAL MODELS FOR SAFETY 35 Assessment of Inflammation in Animal Models (Macroscopic or Histological Inflammation in the Ileum or in the Colon)..... . 349 36 Assessment of Inflammation in Animal Models (Quantification of TNFA, IFNG, IL4, and IL10 mRNAs by Real-Time PCR).... 357 37 Assessment of Inflammation in Animal Models (Quantification of TNF-α, IFN-γ, IL-4, and IL-10 Proteins by ELISA).... . . . . . 367 38 Detection of Myeloperoxidase Activity by Enzyme Linked Immunosorbent Assay............. . . 379 39 Assessment of Bacterial Translocation Through Mesenteric Lymph Nodes [MLN] and Spleen Cultures.........389 Index................ . . . . . . 401 |
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فهرست به فارسی (ترجمه ماشینی): پیشگفتار.................. . . v |
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مشخصات فایل |
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| عنوان (Title): | Biosafety Assessment of Probiotic Potential |
| نام فایل (File name): | 1172-www.GeneProtocols.ir-Biosafety Assessment of Probiotic Potential.pdf |
| عنوان فارسی (Title in Persian): |
ارزیابی ایمنی زیستی پتانسیل پروبیوتیک |
| ایجاد کننده: | Mitesh Kumar Dwivedi, Natarajan Amaresan, A. Sankaranarayanan, Rasheedunnisa Begum |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 9781071625088 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 7.65 مگابایت |
| تعداد صفحات (Book length in pages): | 396 |
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