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فهرست مطالب: Preface...................vContributors................... . xi 1 Quantification of Microtubule-Bundling Activity of MAPs Using TIRF Microscopy.............. 1 2 Actin: Static and Dynamic Studies........... . . . . . 13 3 3D Visualization of Microtubules in Epidermal Pavement Cells..... 25 4 Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells............. . . . . . 43 5 Single-Cell Confinement Methods to Study Plant Cytoskeleton.... 63 6 Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures......... . . 77 7 Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography............ . . . . . 89 8 Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana)...... . . . . . 103 9 Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis........ . . . 113 10 Expansion Microscopy of Plant Cells (PlantExM)....... . . . . . 127 11 Microfluidic Device for High-Resolution Cytoskeleton Imaging and Washout Assays in Physcomitrium (Physcomitrella) patens.... . . 143 12 Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens...... . . . . 159 13 Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools............... . 173 14 Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata..........193 15 Studying Nuclear Dynamics in Response to Actin Disruption in Planta............... . . . 203 16 Cytoskeleton Remodeling in Arabidopsis Stigmatic Cells Following Pollination.............. . . 215 17 Investigation of ROP GTPase Activity and Cytoskeleton Dynamics During Tip Growth in Root Hairs and Pollen Tubes..... 227 18 Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study.. . . . . 237 19 Microtubule Reorganization During ABA-Induced Stomatal Closure in Arabidopsis.............. . . 249 20 Imaging of Cortical Microtubules in Plants Under Salt Stress.... . . 257 21 Analysis of Actin Array Rearrangement During the Plant Response to Bacterial Stimuli............. . . 263 22 Live-Cell Imaging of Cytoskeletal Responses and Trafficking During Fungal Elicitation............. . . . . . 271 23 Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes.......... . . 285 24 Noninvasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings.............. 297 25 Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging...... . . . . . 311 26 Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells............ . . . . 317 27 Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato......... . . . . . 327 28 Light Microscopy Technologies and the Plant Cytoskeleton..... . . . 337 29 Investigating Plant Protein–Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton.......... 353 Index.................... 367 |
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فهرست به فارسی (ترجمه ماشینی): پیشگفتار...................v |
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مشخصات فایل |
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عنوان (Title): | The Plant Cytoskeleton_ Methods and Protocols |
نام فایل (File name): | 1177-www.GeneProtocols.ir-The Plant Cytoskeleton_ Methods and Protocols-Humana Pr.pdf |
عنوان فارسی (Title in Persian): |
اسکلت سلولی گیاهی - روشها و پروتکل ها |
ایجاد کننده: | Patrick J. Hussey, Pengwei Wang |
زبان (Language): | انگلیسی English |
سال انتشار: | 2023 |
شابک ISBN: | 9781071628669 |
نوع سند (Doc. type): | کتاب |
فرمت (File extention): | |
حجم فایل (File size): | 15.5 مگابایت |
تعداد صفحات (Book length in pages): | 368 |
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