فهرست مطالب:

Preface ............ . . v
Contributors........... . . . . ix
PART I ANALYSIS OF THE EPIGENOME: DNA METHYLATION
1 DNA Methylation Analysis Using Bisulfite Pyrosequencing ...3
Ken Higashimoto, Satoshi Hara, and Hidenobu Soejima
2 Post-bisulfite Adaptor Tagging Based on an ssDNA Ligation
Technique (tPBAT) ......... . . . . 21
Fumihito Miura, Yukiko Shibata, Miki Miura,
and Takashi Ito
3 Reduced Representation Bisulfite Sequencing (RRBS).... . . . . 39
Kazuhiko Nakabayashi, Michihiro Yamamura,
Keita Haseagawa, and Kenichiro Hata
PART II ANALYSIS OF THE EPIGENOME: HISTONE MODIFICATION
AND CHROMATIN STRUCTURE
4 Chromatin Immunoprecipitation Sequencing (ChIP-seq) for Detecting
Histone Modifications and Modifiers ....... . 55
Shinjiro Hino, Tetsuya Sato, and Mitsuyoshi Nakao
5 ATAC-Seq Analysis of Accessible Chromatin: From Experimental Steps
to Data Analysis .......... . 65
Mayu Tatara, Taiyo Ikeda, Satoshi H. Namekawa,
and So Maezawa
6 Low-Input CUT&RUN for Mouse Oocytes and Preimplantation
Embryos ........... . . 83
Ryoya Hayashi and Azusa Inoue
7 Imaging Chromatin Accessibility by Assay of Transposase-Accessible
Chromatin with Visualization ........ . 93
Yusuke Miyanari
8 STREAMING-Tag System: Technology to Enable Visualization
of Transcriptional Activity and Subnuclear Localization of Specific
Endogenous Genes.........103
Hiroaki Ohishi and Hiroshi Ochiai
9 Bioinformatics Pipelines for Identification of Super-Enhancers
and 3D Chromatin Contacts ........ . . 123
Akihiko Sakashita, Chikara Takeuchi, So Maezawa,
and Satoshi H. Namekawa
10 A Method for Detection of Somatic LINE-1 Insertions at the Single-Cell
Level from Postmortem Human Brain....... 147
Miki Bundo and Kazuya Iwamoto
11 Solubilization of Mouse Sperm Chromatin for Sequencing Analyses
Using a Chaperon Protein ........ . . . . 161
Yuko Fukuda, Keishi Shintomi, Kosuke Yamaguchi,
Yasuhiro Fujiwara, and Yuki Okada

PART III MANIPULATION OF THE EPIGENOME: PLATFORMS FOR EPIGENOME EDITING

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