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فهرست مطالب: Preface . . . . . . . . . . . . . . vContributors. . . . . . . . . . . . . . . ix 1 A Brief Introduction to Effector-Triggered Immunity . . . . . . . . 1 Thomas A. Kufer and Maria Kaparakis-Liaskos 2 Lambda Red Recombineering in Shigella flexneri . . . . . . . 9 Adrian Herod, Julie Ryu, and John Rohde 3 Using Genomic Deletion Mutants to Investigate Effector-Triggered Immunity During Legionella pneumophila Infection. . . . . 23 Rachelia R. Wibawa, Pengfei Li, Kathleen McCaffrey, and Elizabeth L. Hartland 4 Methods of Bacterial Membrane Vesicle Production, Purification, Quantification, and Examination of Their Immunogenic Functions . . . . . . 43 Natalie J. Bitto and Maria Kaparakis-Liaskos 5 Gene Gun-Mediated Transient Gene Expression for Functional Studies in Plant Immunity . . . . . . . . . 63 Franz Leissing, Anja Reinstadler, Hannah Thieron, € and Ralph Panstruga 6 Host-Induced Gene Silencing Using BPMV on Soybean to Study Genes in the Soybean Rust Fungus Phakopsora pachyrhizi . . . . . 79 Tobias I. Link 7 Virus-Mediated Protein Overexpression (VOX) in Monocots to Identify and Functionally Characterize Fungal Effectors. . . . . . . . . 93 Kostya Kanyuka 8 Time-Resolved Fluorescence Microscopy Screens on Host Protein Subversion During Bacterial Cell Invasion . . . . . . . . 113 Lisa Sanchez, Yuen-Yan Chang, Nora Mellouk, and Jost Enninga 9 Assaying RIPK2 Activation by Complex Formation . . . . . 133 Heidrun Steinle, Kornelia Ellwanger, and Thomas A. Kufer 10 Identification of Novel Endogenous NOD Ligands: Quantitative Analysis of Binding Affinities of NOD1 or NOD2 with Sphingosine-1-Phosphate Using Microscale Thermophoresis. . . . . . . 151 Gang Pei 11 A Surface Plasmon Resonance-Based Strategy to Characterize Interactions of NLR Proteins with Associated Factors . . . . . . . . 161 Jonas Moecking and Matthias Geyer 12 Functional Assessment of Disease-Associated Pyrin Variants. . . 179 Daria Chirita, Yvan Jamilloux, Thomas Henry, and Flora Magnotti 13 An Assay for the Seeding of Homotypic Pyrin Domain Filament Transitions . . . . . . . . . . . 197 Inga V. Hochheiser and Matthias Geyer vii14 Detection of Gasdermin Activation and Lytic Cell Death During Pyroptosis and Apoptosis . . . . . . . . 209 Benjamin Demarco, Saray Ramos, and Petr Broz 15 Visualizing Effector Triggered Immunity in Response to Pore-Forming Toxins by Live-Cell Imaging . . . . . . . . . 239 Seong H. Chow and Thomas Naderer 16 Non-apoptotic Cell Death Control of Neutrophil Extracellular Trap Formation. . . . . . . . . . . . 253 Akshay A. D’Cruz, Maria Ericsson, and Ben A. Croker 17 Analysis of Bacteria-Triggered Inflammasome: Activation in Neutrophils by Immunoblot . . . . . . . . 265 Re´mi Plane`s, Karin Santoni, and Etienne Meunier 18 Quantifying Regulated Mitochondrial Fission in Macrophages . . . . . 281 Syeda Farhana Afroz, Nicholas D. Condon, Matthew J. Sweet, and Ronan Kapetanovic 19 Live Cell Imaging of T Cell Pyroptosis . . . . . . 303 Andreas Linder and Veit Hornung Index . . . . . . . . . . . . . . . 317 مشخصات فایل |
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عنوان (Title): | Effector-Triggered Immunity_ Methods and Protocols |
نام فایل (File name): | 931-www.GeneProtocols.ir-Effector-Triggered Immunity_ Methods and Protocols-Humana Press (2022).pdf |
عنوان فارسی (Title in Persian): | ایمنی تحریک شده توسط افکتور- روشها و پروتکل ها |
ایجاد کننده: | Thomas A. Kufer, Maria Kaparakis-Liaskos |
زبان (Language): | انگلیسی English |
سال انتشار: | 2022 |
شابک ISBN: | 1071624482, 9781071624487 |
نوع سند (Doc. type): | کتاب |
فرمت (File extention): | |
حجم فایل (File size): | 8.04 مگابایت |
تعداد صفحات (Book length in pages): | 316 |
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