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فهرست مطالب: Preface ............ . . vContributors........... . . . . ix PART I HUMAN BRAIN PREPARATIONS 1 Protocol for the Systematic Fixation, Circuit-Based Sampling, and Qualitative and Quantitative Neuropathological Analysis of Human Brain Tissue ......... . 3 Caitlin S. Latimer, Erica J. Melief, Jeanelle Ariza-Torres, Kim Howard, Amanda R. Keen, Lisa M. Keene, Aimee M. Schantz, Trevor M. Sytsma, Angela M. Wilson, Thomas J. Grabowski, Martin Darvas, Kristen Dams O’Connor, Amber L. Nolan, Brian L. Edlow, Christine L. Mac Donald, and C. Dirk Keene 2 Extraction and Purification of Single Nuclei from Frozen Human Brain Tissue ......... . . . 31 Carter R. Palmer and Jerold Chun 3 Isolation of Human Microglia from Neuropathologically Diagnosed Cases in the Single-Cell Era ........ . . . 43 Lih-Fen Lue, Douglas G. Walker, Suet Theng Beh, and Thomas G. Beach 4 Microglia in Human Postmortem Brain Samples: Quantitative Ultrastructural Analysis of Scanning Electron Microscopy Images... 63 Marie-Kim St-Pierre, Eva Sˇimoncˇicˇova´, Micae ¨l Carrier, and Marie-E`ve Tremblay 5 Three-Dimensional Imaging of Fibrinogen and Neurovascular Alterations in Alzheimer’s Disease....... . . . . 87 Mario Merlini, Elif G. Sozmen, Keshav S. Subramanian, Alissa L. Nana, William W. Seeley, and Katerina Akassoglou PART II HUMAN NEURAL CELLULAR MODELS 6 Human-Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer’s Disease ......... . . 105 Jessica E. Young and Lawrence S. B. Goldstein 7 Modeling Alzheimer’s Disease Using Human Brain Organoids ... . . 135 Karina Karmirian, Mariana Holubiec, Livia Goto-Silva, Ivan Fernandez Bessone, Gabriela Vitoria, Beatriz Mello, Matias Alloatti, Bart Vanderborght, Toma´s L. Falzone, and Stevens Rehen vii viii Contents 8 Three-Dimensional Biohybrid StarPEG–Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer’s Disease Pathology.......... 159 Tohid Siddiqui, Hilal Celikkaya, Zeynep Tansu Atasavum, Stanislava Popova, Uwe Freudenberg, Carsten Werner, and Caghan Kizil PART III NUCLEIC ACID ANALYSES OF HUMAN BRAIN 9 Computational Approaches to Assess Abnormal Metabolism in Alzheimer’s Disease Using Transcriptomics .....173 Hatice Bu¨s¸ra Lu¨leci, Dilara Uzuner, Tunahan C¸ akır, and Madhav Thambisetty 10 FISHing for Chromosome Instability and Aneuploidy in the Alzheimer’s Disease Brain.......... . . . . 191 Yuri B. Yurov, Svetlana G. Vorsanova, and Ivan Y. Iourov 11 Somatic CNV Detection by Single-Cell Whole-Genome Sequencing in Postmortem Human Brain ........ . 205 Diego Perez-Rodriguez, Maria Kalyva, Catherine Santucci, and Christos Proukakis PART IV LIPIDS ANALYSES OF HUMAN BRAIN 12 Mass Spectrometry Analysis of the Human Brain Sphingolipidome .. . . . . 233 Xin Ying Chua, Ryan Huang, Deron Herr, Mitchell K. P. Lai, Markus R. Wenk, and Federico Torta 13 Mass Spectrometry for the Advancement of Lipid Analysis in Alzheimer’s Research ......... 245 Jonatan Martı´nez-Gardeazabal, Marta Moreno-Rodrı´guez, Estı´baliz Gonza´lez de San Roma´n, Beatriz Abad, Iva´n Manuel, and Rafael Rodrı´guez-Puertas PART V PROTEIN ANALYSES OF HUMAN BRAIN 14 Use of Affinity Purification–Mass Spectrometry to Identify Phosphorylated Tau Interactors in Alzheimer’s Disease.... . . . 263 Geoffrey Pires, Beatrix Ueberheide, Thomas Wisniewski, and Eleanor Drummond 15 In Vitro Amplification of Pathogenic Tau Seeds from Neurodegenerative Disease Patient Brains ......... . . 279 Hong Xu and Virginia M. -Y. Lee 16 Aβ and Tau Prions Causing Alzheimer’s Disease ..... . . . 293 Carlo Condello, Gregory E. Merz, Atsushi Aoyagi, William F. DeGrado, and Stanley B. Prusiner Index ............ . . . 339 مشخصات فایل |
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| عنوان (Title): | Alzheimers Disease_ Methods and Protocols |
| نام فایل (File name): | 962-www.GeneProtocols.ir-Alzheimers Disease_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): |
بیماری آلزایمر - روشها و پروتکل ها
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| ایجاد کننده: | Jerold Chun |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071626542 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 9.04 مگابایت |
| تعداد صفحات (Book length in pages): | 341 |
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