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فهرست مطالب: Preface........... . . . vContributors........... . . . . ix 1 Guidance on Processing the 10x Genomics Single Cell Gene Expression Assay........... 1 Katharina Danielski 2 BD Rhapsody™ Single-Cell Analysis System Workflow: From Sample to Multimodal Single-Cell Sequencing Data...... . 29 Jannes Ulbrich, Vadir Lopez-Salmeron, and Ian Gerrard 3 Profiling Transcriptional Heterogeneity with Seq-Well S3: A Low-Cost, Portable, High-Fidelity Platform for Massively Parallel Single-Cell RNA-Seq........ . . . . 57 Riley S. Drake, Martin Arreola Villanueva, Mike Vilme, Daniela D. Russo, Andrew Navia, J. Christopher Love, and Alex K. Shalek 4 A MATQ-seq-Based Protocol for Single-Cell RNA-seq in Bacteria.. 105 Christina Homberger, Antoine-Emmanuel Saliba, and Jo ¨rg Vogel 5 Full-Length Single-Cell RNA-Sequencing with FLASH-seq... . . . . . 123 Vincent Hahaut and Simone Picelli 6 Plant Single-Cell/Nucleus RNA-seq Workflow.... . . . . . 165 Sandra Thibivilliers, Andrew Farmer, Susan Schroeder, and Marc Libault 7 Ensuring Quality Cell Input for Single Cell Sequencing Experiments by Viability and Singlet Enrichment Using Cell Sorting... . . . 183 Malte Paulsen 8 Tissue RNA Integrity in Visium Spatial Protocol (Fresh Frozen Samples). 191 Federica Antico, Marta Gai, and Maddalena Arigoni 9 Single-Cell RNAseq Data QC and Preprocessing.... . . . 205 Martina Olivero and Raffaele A. Calogero 10 Single-Cell RNAseq Complexity Reduction..... . . 217 Francesca Cordero and Raffaele A. Calogero 11 Functional-Feature-Based Data Reduction Using Sparsely Connected Autoencoders......... . . . . 231 Luca Alessandri and Raffaele A. Calogero 12 Single-Cell RNAseq Clustering....... . 241 Marco Beccuti and Raffaele A. Calogero 13 Identifying Gene Markers Associated with Cell Subpopulations... . . 251 Maria Luisa Ratto and Luca Alessandri vii viii Contents 14 A Guide to Trajectory Inference and RNA Velocity.... . 269 Philipp Weiler, Koen Van den Berge, Kelly Street, and Simone Tiberi 15 Integration of scATAC-Seq with scRNA-Seq Data.... . . 293 Ivan Berest and Andrea Tangherloni 16 Using “Galaxy-rCASC”: A Public Galaxy Instance for Single-Cell RNA-Seq Data Analysis......... . 311 Pietro Mandreoli, Luca Alessandri, Raffaele A. Calogero, Marco Antonio Tangaro, and Federico Zambelli 17 Bringing Cell Subpopulation Discovery on a Cloud-HPC Using rCASC and StreamFlow......... . . 337 Sandro G. Contaldo, Luca Alessandri, Iacopo Colonnelli, Marco Beccuti, and Marco Aldinucci 18 Profiling RNA Editing in Single Cells...... . 347 Adriano Fonzino, Graziano Pesole, and Ernesto Picardi 19 Practical Considerations for Complex Tissue Dissociation for Single-Cell Transcriptomics....... . 371 Stephanie T. Pohl, Maria Llamazares Prada, Elisa Espinet, and Renata Jurkowska Index........... . . . . 389 مشخصات فایل |
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| عنوان (Title): | Single Cell Transcriptomics_ Methods and Protocols |
| نام فایل (File name): | 982-www.GeneProtocols.ir-Single Cell Transcriptomics_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
ترنسکریپتومیکس تک سلول _ روش ها و پروتکل ها
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| ایجاد کننده: | Raffaele A. Calogero, Vladimir Benes |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071627556 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 10.4 مگابایت |
| تعداد صفحات (Book length in pages): | 394 |
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برچسب ها:
2023,
انگلیسی,
اومیکس,
ترنسکریپتوم,
سلول شناسی,
سیتوژنتیک,
پروتکل سلولی مولکولی,
پروتکل ژنتیک,
