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فهرست مطالب: Preface to the Second Edition ......... . . vContributors........... . . . . ix PART I BACKGROUND INFORMATION 1 A Beginner’s Guide to T Cell Development ...... . 3 Re´my Bosselut 2 Revelations in Thymic Epithelial Cell Biology and Heterogeneity from Single-Cell RNA Sequencing and Lineage Tracing Methodologies .. 25 Abigail Morales-Sanchez, Susannah C. Shissler, Jennifer E. Cowan, and Avinash Bhandoola 3 Early Development of Innate Lymphoid Cells .....51 Yi Ding, Christelle Harly, Arundhoti Das, and Avinash Bhandoola 4 Development of γδ T Cells: Soldiers on the Front Lines of Immune Battles .........71 Alejandra V. Contreras and David L. Wiest 5 Principles of Advanced Flow Cytometry: A Practical Guide ...89 Shafiuddin Siddiqui and Ferenc Liva´k PART II CORE APPROACHES AND STRATEGIES 6 Genetic Strategies to Study T Cell Development..... . . . 117 Re´my Bosselut 7 A Simplified Approach to Evaluating T Cell Development by Flow Cytometry ......... . . . . 131 Jan Y. M. Lee and Paul E. Love 8 Purification of Thymocyte and T Cell Subsets .....151 Andrea C. Carpenter, Laura B. Chopp, Jong Kyong Kim, and Re´my Bosselut 9 Generation of Bone Marrow Chimeras ......165 Yayi Gao and Re´my Bosselut 10 Generation of Murine T Cell Effector Populations In Vitro ...173 Melanie S. Vacchio PART III SPECIFIC TECHNIQUES 11 Large-Scale Isolation of Mouse Thymic Epithelial Cells.... . . . 189 Izumi Ohigashi, Mami Matsuda-Lennikov, and Yousuke Takahama vii viii Contents 12 Generation of Retrogenic Mice to Investigate T Cell Development .. . . . . 199 Yayi Gao and Re´my Bosselut 13 Purification of Bone Marrow Precursors to T Cells and ILCs... . . . . 211 Devin Kenney and Christelle Harly 14 Studying T Cell Development in Neonatal and Adult Thymic Slices.. . . . . 233 Laura L. McIntyre, Lydia K. Lutes, and Ellen A. Robey 15 Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines ....... . . 249 Mahmood Mohtashami, Patrick M. Brauer, and Juan Carlos Zu´ n˜iga-Pflu¨cker 16 Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements ......... . . 261 Danielle J. Dauphars, Glendon Wu, Craig H. Bassing, and Michael S. Krangel 17 Ultrasound-Guided Intra-thymic Cell Injection ..... . . . 283 Hristo Georgiev, Laura B. Chopp, and Kristin A. Hogquist 18 Fetal Thymic Organ Culture and Negative Selection ....293 Emma Teixeiro and Mark A. Daniels 19 In Vitro Analysis of Thymocyte Signaling ...... . . . 303 Emma Teixeiro and Mark A. Daniels 20 Identification and Purification of Human T Cell Precursors ...315 Inge Van de Walle, Nina Lambrechts, Anaı ¨s Derveeuw, Marieke Lavaert, Juliette Roels, and Tom Taghon 21 In Vitro Model Systems to Study Human T Cell Development ... . . 335 Nina Lambrechts, Kai Ling Liang, Imke Velghe, Steven Strubbe, Anne-Catherine Dolens, and Tom Taghon 22 Zebrafish: A Tractable Model for Analysis of T Cell Development ..355 Robert Sertori, Yong Zhang, and David L. Wiest Index ............ . . . 379 مشخصات فایل |
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| عنوان (Title): | T-Cell Development_ Methods and Protocols |
| نام فایل (File name): | 978-www.GeneProtocols.ir-T-Cell Development_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
توسعه سلولهای T - روشها و پروتکل ها
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| ایجاد کننده: | Remy Bosselut, Melanie S. Vacchio |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071627396 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 14.4 مگابایت |
| تعداد صفحات (Book length in pages): | 382 |
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