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فهرست مطالب: Preface...................vContributors................... . ix Contents PART I A GROWING HETEROGENEITY OF T CELL SUBSETS 1 Ligand Identification for Orphan MHC-Agnostic T-Cell Receptors by Whole Genome CRISPR–Cas9 Screening........ . . . 3 Michael D. Crowther, Mateusz Legut, and Andrew K. Sewell 2 Discovery of HLA-E-Presented Epitopes: MHC-E/Peptide Binding and T-Cell Recognition............... 15 Paula Ruibal, Kees L. M. C. Franken, Krista E. van Meijgaarden, Lucy C. Walters, Andrew J. McMichael, Geraldine M. Gillespie, Simone A. Joosten, and Tom H. M. Ottenhoff 3 Identification of Human Antigen-Specific T Cells Using Class II MHC Tetramer Staining and Enrichment.........31 Yi-Gen Pan and Laura F. Su 4 Characterization of KIR+CD8+ Regulatory T Cells in Humans by scRNA- and TCR-seq.............. . . 41 Jing Li, Julie Wilhelmy, and Mark M. Davis 5 Detection of α-Synuclein-Specific T Cells in Parkinson’s Disease....123 Rekha Dhanwani, Alessandro Sette, and Cecilia S. Lindestam Arlehamn PART II TCR REPERTOIRE SEQUENCING AND ANTIGEN ANALYSIS 6 The Intra-Tumoral T Cell Receptor Repertoire: Steps Towards a Useful Clinical Biomarker............ . . . . 135 Gayathri Nageswaran, Suzanne Byrne, Selvaraju Veeriah, and Benny Chain 7 Enriching and Characterizing T Cell Repertoires from 30 Barcoded Single-Cell Whole Transcriptome Amplification Products.....159 Tasneem Jivanjee, Samira Ibrahim, Sarah K. Nyquist, G. James Gatter, Joshua D. Bromley, Swati Jaiswal, Bonnie Berger, Samuel M. Behar, J. Christopher Love, and Alex K. Shalek 8 Tetramer-Associated T Cell Receptor Sequencing.......183 Michael Malone, Ke-Yue Ma, Shu-Qi Zhang, and Ning Jiang 9 T-Cell Repertoire Characterization........... . . . . 209 Anna Pasetto and Marcus Buggert 10 Characterization of Mouse CD4 TCR and Its Targeting Antigen...221 Wenhua Liang and Feng Wang vii viii Contents 11 Rapid Identification of MHCII-Binding Peptides Through Microsphere-Assisted Peptide Screening (MAPS).......233 Luke F. Bugada, Mason R. Smith, and Fei Wen 12 A High-Throughput Strategy for T-Cell Receptor Cloning and Expression................ . 251 Qiong Xia, Huang Huang, and Mark M. Davis PART III COMPUTATIONAL TOOLS FOR TCR REPERTOIRE ANALYSIS 13 Epitope-Specific T Cell Receptor Data and Tools in the Immune Epitope Database...............267 William Chronister, Alessandro Sette, and Bjoern Peters 14 A Bioinformatic Framework for Dissecting the Dynamics of T Cells from Single-Cell Transcriptome.......... 281 Lei Zhang and Jiesheng Li 15 Grouping T-Cell Antigen Receptors by Specificity...... . . . . . 291 Chunlin Wang, Huang Huang, and Mark M. Davis 16 Flexible Distance-Based TCR Analysis in Python with tcrdist3..... . 309 Koshlan Mayer-Blackwell, Andrew Fiore-Gartland, and Paul G. Thomas 17 Multimodal T Cell Analysis with CoNGA......... . . . . . 367 Stefan A. Schattgen, William D. Hazelton, Paul G. Thomas, and Philip Bradley Index.................... 389 مشخصات فایل |
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| عنوان (Title): | T-Cell Repertoire Characterization |
| نام فایل (File name): | 973-www.GeneProtocols.ir-T-Cell Repertoire Characterization-Humana (2022).pdf |
| عنوان فارسی (Title in Persian): |
خصوصیات رپرتوار سلولهای T
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| ایجاد کننده: | Huang Huang (editor), Mark M. Davis (editor) |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 9781071627112 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 10 مگابایت |
| تعداد صفحات (Book length in pages): | 395 |
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