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قابل دانلود از جمعه, ۲۲ ارديبهشت ۱۴۰۲
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فهرست مطالب: Preface . . . . . . . . . . . . . . vContributors . . . . . . . . . . . . . . . xi PART I MODEL SYSTEMS USED TO STUDY GOLGI ORGANIZATION AND FUNCTION 1 Live Cell Imaging of Yeast Golgi Dynamics . . . . . . . 3 Giancarlo Costaguta, Gregory S. Payne, and Lydia Daboussi 2 Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuole . . . . . . . . . . 17 Mariana Jimenez, Jordan T. Best, Swapneeta S. Date, and Todd R. Graham 3 Immunofluorescence as a Method to Study Golgi Organization in Larval Salivary Glands of Drosophila melanogaster . . . . . 29 Tetyana Chorna and Julie A. Brill 4 Microscopy and Immunocytochemistry-Based Methods to Study Cell Wall Biosynthetic Enzymes in the Golgi . . . . . 39 Sang-Jin Kim and Federica Brandizzi 5 Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells . . . . . . . . 53 Graham Warren 6 Generation of GM130 Conditional Knockout Mouse . . . . . . . . 61 Mei Mei and Shilai Bao 7 Monitoring Effects of Membrane Traffic Via Changes in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts . . . . . . . . . 83 Maha M. Hamed, Kenichiro Taniguchi, and Mara C. Duncan PART II MORPHOLOGICAL STUDIES OF GOLGI ORGANIZATION AND FUNCTION 8 Immunofluorescence Microscopy of the Mammalian Golgi Apparatus . . . . 101 Maryam Arab, Sanjeev Chavan Nayak, Teresa Vitali, and Martin Lowe 9 Studying the Organization of the Golgi by Super-Resolution Microscopy . 113 Hieng Chiong Tie and Lei Lu 10 Super-Resolution Live Imaging of Cargo Traffic Through the Golgi Apparatus in Mammalian Cells . . . . . . . . 127 Takuro Tojima, Daisuke Miyashiro, Yasuhito Kosugi, and Akihiko Nakano viiviii Contents 11 Studying Golgi Structure and Function by Thin Section TEM . . . . . 141 Helena Vihinen and Eija Jokitalo 12 Algorithm for Modern Electron Microscopic Examination of the Golgi Complex . . . . . . . . . 161 Alexander A. Mironov and Galina V. Beznoussenko 13 High-Pressure Freezing Followed by Freeze Substitution: An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking . . . . . . . . . . 211 Shijie Liu, Irina D. Pokrovskaya, and Brian Storrie 14 Negative Staining Electron Microscopy for Morpho-Functional Characterization of Purified Golgi Membranes from Mammalian Cells . . . 225 Stefano De Tito, Gabriele Turacchio, and Carmen Valente 15 An Electron Tomographic Analysis of Giantin-Deficient Golgi Proposes a New Function of the Golgin Protein Family . . . . . . 235 Ayano Satoh, Mitsuko Hayashi-Nishino, and Kunihiko Nishino 16 Modeling the Cryo-EM Structure of the Exocyst Complex . . . 247 Kunrong Mei and Wei Guo 17 Quantitatively Assessing Co-Localization of Golgi Proteins by Distance Analysis Using the DiAna Software . . . . . . 263 Nina Freimuth and Michael Sauer 18 A Primer on Deep Learning-Based Cellular Image Classification of Changes in the Spatial Distribution of the Golgi Apparatus After Experimental Manipulation . . . . . . . . . . . 275 Daisuke Takao, Yuki M. Kyunai, Yasushi Okada, and Ayano Satoh PART III BIOCHEMICAL AND IN VITRO RECONSTITUTION ASSAYS IN GOLGI STUDIES 19 Reconstitution of Vesicle Budding from the TGN and Immunoisolation of Vesicles Enriched with a Specific Cargo Client . . . . . . 289 Mo Wang, Jinhui Wang, and Yusong Guo 20 Common Assays in Mammalian Golgi Studies . . . . . 303 Jie Li, Jianchao Zhang, Sarah Bui, Erpan Ahat, Divya Kolli, Whitney Reid, Lijuan Xing, and Yanzhuang Wang 21 In Vitro Methods to Investigate the Disassembly of the Golgi Ribbon During the G2-M Transition of the Cell Cycle . . . . . . . 333 Inmaculada Ayala and Antonino Colanzi 22 Generation and Analysis of hTERT-RPE1 VPS54 Knock-Out and Rescued Cell Lines . . . . . . . . . . 349 Amrita Khakurel, Tetyana Kudlyk, and Vladimir V Lupashin 23 Rapid COG Depletion in Mammalian Cell by Auxin-Inducible Degradation System . . . . . . . . . . . . . 365 Farhana Taher Sumya, Irina D. Pokrovskaya, and Vladimir V LupashinContents ix 24 Generation of Stable Cell Lines Expressing Golgi Reassembly Stacking Proteins (GRASPs) by Viral Transduction . . . . . 391 Sarah Bui, Jie Li, Drew Stark, Ali Houmani, and Yanzhuang Wang 25 Quantitative Proteomics Analysis of Purified Rat Liver Golgi . . . . . . . 417 Xuequn Chen and Yanzhuang Wang 26 Comparative Genomics for Evolutionary Cell Biology Using AMOEBAE: Understanding the Golgi and Beyond . . . . . 431 Lael D. Barlow, William Maciejowski, Kiran More, Kara Terry, Romana Vargova´ , Kristı´na Za´ honova´ , and Joel B. Dacks 27 Common Markers and Small Molecule Inhibitors in Golgi Studies . . 453 Sarah Bui, Drew Stark, Jie Li, Jianchao Zhang, and Yanzhuang Wang PART IV MECHANISM OF GOLGI STRUCTURE FORMATION AND MEMBRANE TRAFFICKING 28 Visualizing Reversible Cisternal Stacking in Budding Yeast Pichia pastoris . 497 Roma Dahara, Bhawik Jain, and Dibyendu Bhattacharyya 29 Methods for Studying Membrane-Proximal GAP Activity on Prenylated Rab GTPase Substrates . . . . . . . . . . 507 Carolyn M. Highland, Laura L. Thomas, and Christopher J. Fromme 30 Studying the Role of Lipid Geometry in COPI Vesicle Formation . . 519 Hyewon Choi, Kunyou Park, Victor W. Hsu, and Seung-Yeol Park 31 Unbiased Quantification of Golgi Scattering and Golgi–Centrosome Association . . . . . . . . . . . 529 Keyada B. Frye, Xiaodong Zhu, Alexey Khodjakov, and Irina Kaverina 32 Detection and Analysis of Microtubule Nucleator γ-Tubulin Ring Complex . . . . . . . . . . . . . 543 Franco K. C. Au, Khoi T. D. Le, and Robert Z. Qi 33 Quantification of Golgi Entry and Exit Kinetics of Protein Cargoes . . . . . . 559 Jingqi Wang, Ellie Hyun-Jung Cho, Paul A. Gleeson, and Lou Fourriere 34 CARTS Formation Assay . . . . . . . . 573 Yuichi Wakana and Mitsuo Tagaya 35 Quantification of Protein Exit at the Trans-Golgi Network . . . 583 Mai Ly Tran, Yeongho Kim, and Julia von Blume 36 Endosomal Transport to Lysosomes and the Trans-Golgi Network in Neurons and Other Cells: Visualizing Maturational Flux . . . 595 Ryan J. Mulligan, Chan Choo Yap, and Bettina Winckler 37 Immunoprecipitation and Western Blot Analysis of AP-1 Clathrin-Coated Vesicles . . . . . . . . . 619 Margaret Kell, Abby Halpern, and Heike Fo ¨lsch 38 Live Imaging of Golgi Outposts in Drosophila Dendritic Arbors . . . 635 Josephine W. Mitchell and Jill Wildonger 39 Targeted Protein Unfolding at the Golgi Apparatus . . . . . 645 Jasmin Schillinger and Doris Hellerschmiedx Contents PART V GOLGI FUNCTIONS IN PHYSIOLOGY AND PATHOLOGY 40 Modeling N-Glycosylation: A Systems Biology Approach for Evaluating Changes in the Steady-State Organization of Golgi-Resident Proteins . . . . . . . . . 663 Rebecca Morgan, Ben West, A. Jamie Wood, and Daniel Ungar 41 Simple N-Glycan Profile Analysis Using Lectin Staining, Mass Spectrometry, and GlycoMaple . . . . . . . . 691 Wei-Wei Ren and Morihisa Fujita 42 In Vitro Methods to Study the Golgi Apparatus Role in Proteoglycan and Glycosaminoglycan Synthesis . . . . . . . . . . . 709 Kristian Prydz 43 Analysis of Golgi Protein Acetylation Using In Vitro Assays and Parallel Reaction Monitoring Mass Spectrometry . . . . . . . . 721 Dea Slade and Markus Hartl 44 Identification and Characterization of GRASP55 O-GlcNAcylation . . . . . . 743 Xiaoyan Zhang 45 Assaying Sterol-Regulated ER-to-Golgi Transport of SREBP Cleavage-Activating Protein Using Immunofluorescence Microscopy . . . . 755 Chiaki T. Ishida, Wei Shao, and Peter J. Espenshade 46 Analysis of Golgi Morphology Using Immunofluorescence and CellProfiler Software . . . . . . . . . . . . . 765 Isabel Mejia, Yu-Chuan Chen, and Begon˜a Dı´az 47 Analysis of Golgi Secretory Functions in Cancer . . . . . . . . 785 Priyam Banerjee, Xiaochao Tan, William K. Russell, and Jonathan M. Kurie Index . . . . . . . . . . . . . . . 811 مشخصات فایل |
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| عنوان (Title): | Golgi_ Methods and Protocols |
| نام فایل (File name): | 958-www.GeneProtocols.ir-Golgi_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): |
دستگاه گلژی - روشها و پروتکل ها
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| ایجاد کننده: | Yanzhuang Wang, Vladamir V. Lupashin, Todd R. Graham |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071626382, 9781071626399 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 27.9 مگابایت |
| تعداد صفحات (Book length in pages): | 809 |
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انگلیسی,
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فیزیولوژی پزشکی,
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گلژی,
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