فهرست مطالب:

Foreword. . . . . . . . . . . . . . . . . . . . . . . . . . v
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Contributors. . .  . . . . . . . . . . . . . . . . . . . xiii
1 Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine . . . . . . . 1
2 Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis . . . . . 25
3 Rice Gene Knockout or Downregulation through CRISPR-Cas9 . . . . . . . . . . . . . 35
4 CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome . . . 51
5 CRISPR-Cas9-Mediated Genome Editing of the Model Grass Species Brachypodium distachyon . . . . 63
6 CRISPR-Cas9-Mediated Gene Editing of the Plant Pathogenic Oomycete Phytophthora palmivora. . . . 87
7 An Effective CRISPR-Cas9 Technology for Efficiently Isolating Transgene-Free Mutants in Arabidopsis, Brassica napus, Strawberry, and Soybean . . . . 99
8 An Optimized RNA-Guided Cas9 System for Efficient Simplex and Multiplex Genome Editing in Barley (Hordeum vulgare L.) . . . . . . 117
9 Genome Editing of Mammalian Cells Using CRISPR-Cas: From In Silico Designing to In-Culture Validation . . . . 143
10 Cloning-Free (DNA-Free) CRISPR-Cas9-Mediated Gene Editing in Human Liver Cell Line and Its Detection . . . . 163
11 A Procedure to Design Guide RNA, Assemble Fragments, and Detect Mutation for Genome Editing in Flax. . . . . 173
12 Delivery of Cas9/sgRNA RNP into Wheat Microspores Using Synthetic CPP for Genome Editing and Gene Expression Modulation . . . . 191
13 CRISPR-Cas9-Mediated Gene Editing in Wheat: A Step-by-Step Protocol . . . . . 203
14 CRISPR-Cas9-Based Genome Editing of Banana . . . . . . . . . . . . . . . . . . . . . . . . . . . 223
15 Homology-Directed Transgene-Free Gene Editing in Chlamydomonas reinhardtii . . . . . 237
Glossary . . . . . . .  . . 253
Index . . . . . . 273

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