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فهرست مطالب: Preface...................vContributors................... . ix 1 Imaging Mass Cytometry in Immuno-Oncology........1 Tiziana Cotechini, Oliver Jones, and Charles Colin Thomas Hindmarch 2 Analyzing the Tumor-Immune Microenvironment by Flow Cytometry.. . 17 Franc¸ois Santinon, Yoon Kow Young, Sonia V. del Rincon, and Koren K. Mann 3 Blood Sample Processing and Banking for Functional and Molecular Analyses.............. . 37 Pame´la The´bault, Jean-Franc¸ois Cailhier, and Re´jean Lapointe 4 Quantitatively Assessing the Respiratory Burst in Innate Immune Cells... 47 Ava L. Sargent, Jordan A. Leedberg, Jessica E. Burress, Purva S. Dalwadi, Kevin S. O’Fallon, Erin Gaffney-Stomberg, and Peter C. W. Gaines 5 Visualizing NETosis Using a Novel Neutrophil Extracellular Trap-Specific Marker.............. . . . 71 Meghan L. De Meo, Muhammad H. Shahzad, and Jonathan D. Spicer 6 In Vitro Methods to Evaluate Macrophage Polarization and Function in Cancer............... 81 Aldo Ummarino, Cle´ment Anfray, Akihiro Maeda, Fernando Torres Andon, and Paola Allavena 7 In Vivo Analysis of Tumor-Associated Macrophages in the Tumor Microenvironment.............. . . . . . 93 Cle´ment Anfray, Aldo Ummarino, Alfonso Calvo, Paola Allavena, and Fernando Torres Andon 8 Assessing the Immune Regulatory Role of Metabolites In Vitro.... 109 Sarah McPhedran, Junu Choe, Sarah MacPherson, Gabrielle Kowalchuk, Vanessa Chan, Marisa K. Kilgour, and Julian J. Lum 9 Mouse Models of Obesity to Study the Tumor-Immune Microenvironment.............. . . . . . 121 Sarah Petrecca and Daniela F. Quail 10 Generation and Quantification of Cytotoxic Lymphocytes Following Oncolytic Virus Infection of Multi-cellular Tumor Spheroids..... . . 139 Victoria A. Jennings and Carolina S. Ilkow 11 Assessing the Efficacy of Immune Checkpoint Inhibitors in Preclinical Tumor Models................ . 151 Celia Jacoberger-Foissac, Bertrand Allard, David Allard, and John Stagg 12 Fibroblast Isolation from Mammary Gland Tissue and Syngeneic Murine Breast Cancer Models.............. . . 171 Margarita Bartish, Julian Smith-Voudouris, and Sonia V. del Rincon 13 Multiscale Label-Free Imaging of Fibrillar Collagen in the Tumor Microenvironment.............. . . . . . 187 Michael S. Nelson, Yuming Liu, Helen M. Wilson, Bin Li, Ivan M. Rosado-Mendez, Jeremy D. Rogers, Walter F. Block, and Kevin W. Eliceiri 14 In Situ Measurement of Intra-tumoral Tissue Rigidity....... . 237 Benjamin E. Campbell, Stephanie Mok, and Christopher Moraes 15 Extracellular Matrix Glycation and Crosslinking in Mammary Tumor Progression................ . . . . 247 Jason J. Northey and Valerie M. Weaver 16 Proteomic Assessment of the Murine Mammary Gland Extracellular Matrix.................. . . 261 Samuel E. J. Preston, Vincent R. Richard, Sonia V. del Rincon, Christoph H. Borchers, and Rene´ P. Zahedi 17 Spheroids as a 3D Model of the Hypoxic Tumor Microenvironment.. . . . 273 Sarah M. Kirsh, Sydney A. Pascetta, and James Uniacke 18 Tumor pH Imaging Using Chemical Exchange Saturation Transfer (CEST)-MRI................. . . 287 Dario Livio Longo, Elisa Pirotta, Riccardo Gambino, Feriel Romdhane, Antonella Carella, and Alessia Corrado 19 Profiling the Epigenetic Landscape of the Tumor Microenvironment Using Chromatin Immunoprecipitation Sequencing...... . . . 313 Marina Fukano, Gabriel Alzial, Raphae ¨lle Lambert, and Genevie`ve Deblois 20 Analysis of Spatial Molecular Data.......... . . . . . 349 Alona Levy-Jurgenson, Xavier Tekpli, Vessela N. Kristensen, and Zohar Yakhini 21 Laser-Assisted Single-Cell Labeling and Capture........357 Nicolas Desjardins-Lecavalier, Graziana Modica, and Santiago Costantino 22 3D Hydrogel Cultures for High-Throughput Drug Discovery.... . 369 Karen Sperle, Darrin J. Pochan, and Sigrid A. Langhans 23 Intravital Microscopy of the Metastatic Pulmonary Environment...383 Liane Babes, Bryan George Yipp, and Donna Lorraine Senger 24 A CRISPR Platform for Targeted In Vivo Screens...... . . . . . 397 Vincent Maranda, Yue Zhang, Frederick S. Vizeacoumar, Andrew Freywald, and Franco J. Vizeacoumar Index.................... 411 مشخصات فایل |
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| عنوان (Title): | The Tumor Microenvironment_ Methods and Protocols |
| نام فایل (File name): | 1001-www.GeneProtocols.ir-The Tumor Microenvironment_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
ریزمحیط تومور - روش ها و پروتکل ها
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| ایجاد کننده: | Josie Ursini-Siegel |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071629130 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 16.1 مگابایت |
| تعداد صفحات (Book length in pages): | 417 |
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