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فهرست مطالب: Preface .............. . . . vContributors.............ix PART I INTRODUCTORY REVIEWS 1 Introduction and Classification of Leukemias....... 3 Geoffrey Brown 2 Leukemia Stem Cells: Concept and Implications...... . . 25 Isidro Sa´nchez-Garcı´a and Ce´sar Cobaleda 3 Leukemia Stem Cell Drug Discovery ........ . . 39 Ce´sar Cobaleda and Isidro Sa´nchez-Garcı´a PART II PROTOCOLS 4 Analysis and Isolation of Mouse Leukemic Stem Cells ..... . . 51 Fang Dong, Haitao Bai, and Hideo Ema 5 Mass Cytometry of Hematopoietic Cells .......65 Astraea Jager, Jolanda Sarno, and Kara L. Davis 6 PCR Technology to Identify Minimal Residual Disease ..... . 77 Giovanni Cazzaniga, Simona Songia, and Andrea Biondi 7 Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies....... . 95 Michaela Kotrova, Nikos Darzentas, Christiane Pott, and Monika Bru¨ggemann 8 Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology) ........ . . 113 Daniel Hein, Arndt Borkhardt, and Ute Fischer 9 Single-Cell Transcriptomic Analysis of Hematopoietic Cells .... . . 135 Paulina M. Strzelecka, Anna M. Ranzoni, and Ana Cvejic 10 In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics............. 159 Yiran Liang, Thy Truong, Ying Zhu, and Ryan T. Kelly 11 In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation............ . 181 Kyomi J. Igarashi and Ryo Yamamoto 12 Experimental Competitive Bone Marrow Transplant Assays .... . . 195 Roxann He´tu-Arbour, Sarah Bouali, and Krista M. Heinonen 13 Human T-ALL Xenografts .......... . 215 Patricia Fuentes, Marı´a L. Toribio, and Sara Gonza´lez-Garcı´a vii14 An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts ...... . . 241 Thalia Vlachou, Marco S. Nobile, Chiara Ronchini, Daniela Besozzi, and Pier Giuseppe Pelicci 15 Leukemic Stem Cell Culture in Cytokine-Free Medium ..... 259 Xiaolei Liu and Peter S. Klein 16 Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid .......... . . . 267 Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, and Ronald Hoffman 17 Isolation, Culture, and Manipulation of Human Cord Blood Progenitors.. 281 Cristina Prieto, Damia Romero-Moya, and Rosa Montes 18 Lentiviral Transduction for Optimal LSC/HSC Manipulation.... 299 Gustavo Mostoslavsky 19 Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes .307 Yu Wei Zhang, Julian Mess, and Nina Cabezas-Wallscheid 20 Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes ..317 Sabrina Jacobs, Leonid V. Bystrykh, and Mirjam E. Belderbos 21 Arrayed Molecular Barcoding of Leukemic Stem Cells..... . . 345 Marion Chapellier and Marcus Jara € ˚s 22 In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis............ . 361 Carolina Vicente-Duen˜as 23 In Situ Hematopoietic Stem Cell Imaging ....... . . 373 Aparna Venkatraman, Sarah E. Smith, Sandra Pinho, Meng Zhao, Linheng Li, and Paul Frenette 24 A Genome Editing System for Therapeutical Targeting of Stem Cells .. . . . 383 Giacomo Frati and Annarita Miccio 25 Method for the Generation of Induced Hematopoietic Stem Cells... . 399 Leonid Olender, Klil Levy, and Roi Gazit 26 Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells........... 411 Andre´ G. Deslauriers, Andriana G. Kotini, and Eirini P. Papapetrou 27 High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells..........423 Lorenzo Veschini, Heba Sailem, Disha Malani, Vilja Pietiainen, € Ana Stojiljkovic, Erika Wiseman, and Davide Danovi 28 Bioinformatic Methods to Identify Mutational Signatures in Cancer ..447 S. M. Ashiqul Islam and Ludmil B. Alexandrov Index ..............475 مشخصات فایل |
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| عنوان (Title): | Leukemia Stem Cells_ Methods and Protocols |
| نام فایل (File name): | 969-www.GeneProtocols.ir-Leukemia Stem Cells_ Methods and Protocols-Humana (2021).pdf |
| عنوان فارسی (Title in Persian): |
سلول های بنیادی سرطان خون - روشها و پروتکل ها
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| ایجاد کننده: | César Cobaleda, Isidro Sánchez-García |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2021 |
| شابک ISBN: | 9781071608098 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 12.7 مگابایت |
| تعداد صفحات (Book length in pages): | 481 |
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