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فهرست مطالب: Preface .. .. .. .. .. .. .. .. . . . . . v 2 Establishing a New Research Axolotl Colony .. .. .. .27 3 Health Monitoring for Laboratory Salamanders .. .. .. . . . . 41Marcus J. Crim and Marcia L. Hart 4 Husbandry, Captive Breeding, and Field Survey of Chinese Giant Salamander (Andrias davidianus) .. .. .. .. . . . 75 Wansheng Jiang, Haifeng Tian, and Lu Zhang PART II TRADITIONAL MOLECULAR TECHNIQUES 5 Whole-Mount In Situ Hybridization (WISH) for Salamander Embryos and Larvae .. .. .. .. .. .. . . . . 95 Sruthi Purushothaman and Ashley W. Seifert 6 Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue. .. .. .. . . . . 109 Alex M. Lovely, Timothy J. Duerr, David F. Stein, Evan T. Mun, and James R. Monaghan 7 Ethyl Cinnamate-Based Tissue Clearing Strategies .. .. .. . . 123 Wouter Masselink and Elly M. Tanaka 8 Induction and Characterization of Cellular Senescence in Salamanders .. 135 Qinghao Yu, Hannah E. Walters, and Maximina H. Yun 9 Methods for Studying Appendicular Skeletal Biology in Axolotls. .. . . . . . 155 Camilo Riquelme-Guzma´n and Tatiana Sandoval-Guzma´n 10 Fluorescence In Situ Hybridization of DNA Probes on Mitotic Chromosomes of the Mexican Axolotl .. .. .. . . . . . 165 Melissa Keinath and Vladimir Timoshevskiy 11 The Use of Small Molecules to Dissect Developmental and Regenerative Processes in Axolotls .. .. .. .. .. 175 Ste´phane Roy 12 COMET Assay for Detection of DNA Damage During Axolotl Tail Regeneration. .. .. .. .. .. .183 Belfran Carbonell, Jennifer A´ lvarez, Gloria A. Santa-Gonza´lez, and Jean Paul Delgado PART III EXPERIMENTAL MANIPULATIONS AND SURGERIES 13 In Vivo and Ex Vivo View of Newt Lens Regeneration .. .. . . . . . . 197 Georgios Tsissios, Anthony Sallese, Weihao Chen, Alyssa Miller, Hui Wang, and Katia Del Rio-Tsonis 14 Bead Implantation and Delivery of Exogenous Growth Factors. .. . . . . . . 209 Rena Kashimoto, Saya Furukawa, Sakiya Yamamoto, and Akira Satoh 15 The Accessory Limb Model Regenerative Assay and Its Derivatives .. . . . 217 Michael Raymond and Catherine D. Mccusker 16 Embryonic Tissue and Blastema Transplantations .. .. .. . . . 235 Maritta Schuez, Thomas Kurth, Joshua D. Currie, and Tatiana Sandoval-Guzma´n 17 Retinoic Acid–Induced Limb Duplications. .. .. .. .. 249 Malcolm Maden and Trey Polvadore 18 Isolation and Characterization of Peritoneal Macrophages from Salamanders .. .. .. .. .. .. .259 Anthony Sallese, Georgios Tsissios, J. Rau´ l Pe´rez-Estrada, Arielle Martinez, and Katia Del Rio-Tsonis PART IV BIOINFORMATICS AND GENOMICS 19 Navigation and Use of Custom Tracks within the Axolotl Genome Browser .. .. .. .. .. .. .273 Sergej Nowoshilow and Elly M. Tanaka 20 Chromosome Conformation Capture for Large Genomes .. .. . . . 291 Akane Kawaguchi and Elly M. Tanaka PART V TRANSGENICS AND LINEAGE-TRACING 21 Axolotl Transgenesis via Injection of I-SceI Meganuclease or Tol2 Transposon System. .. .. .. .. .. .. 321 Maritta Schuez and Tatiana Sandoval-Guzma´n 22 A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls .. . . 335 Konstantinos Sousounis, Katharine Courtemanche, and Jessica L. Whited 23 Applying a Knock-In Strategy to Create Reporter-Tagged Knockout Alleles in Axolotls .. .. .. .. .. . . . . . 351 Liqun Wang, Yan-Yun Zeng, Yanmei Liu, and Ji-Feng Fei 24 Baculovirus Production and Infection in Axolotls .. .. .. . . . 369 Prayag Murawala, Catarina R. Oliveira, Helena Okulski, Maximina H. Yun, and Elly M. Tanaka 25 Cell Dissociation Techniques in Salamanders .. .. .. .389 Gabriela Johnson, Nadjib Dastagir, Zachary Beal, Andrew Hart, and James Godwin Contents ix PART VI PHYSIOLOGICAL AND ORGANISMAL TECHNIQUES 26 Axolotl Metabolism: Measuring Metabolic Rate .. .. .. . . . . 407 Moshe Khurgel 27 Artificial Insemination in Axolotl .. .. .. .. .. . 417 Yuka Taniguchi-Sugiura and Elly M. Tanaka 28 Screening Salamanders for Symbionts .. .. .. .. . . . . . 425 Elli Vickers and Ryan Kerney 29 Assessing Leukocyte Profiles of Salamanders and Other Amphibians: A Herpetologists’ Guide .. .. .. .. .. .. . 443 Andrew K. Davis and John C. Maerz PART VII EPILOGUE 30 Best Practices to Promote Data Utility and Reuse by the Non-Traditional Model Organism Community. .. .. .. . . . . 461 Garrett S. Dunlap and Nicholas D. Leigh 31 Now that We Got There, What Next? .. .. .. .. . . . . . 471 Elly M. Tanaka Index .. .. .. .. .. .. .. .. . . . . . . 481 مشخصات فایل |
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| عنوان (Title): | Salamanders_ Methods and Protocols |
| نام فایل (File name): | 963-www.GeneProtocols.ir-Salamanders_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): |
سمندر - روشها و پروتکل ها
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| ایجاد کننده: | Ashley W. Seifert, Joshua D. Currie |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071626580 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 15.9 مگابایت |
| تعداد صفحات (Book length in pages): | 471 |
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