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فهرست مطالب: Preface . . . . . . . . . . . . . . . vContributors. . . . . . . . . . . . . . . . . . ix 1 Starting a C. elegans Research Laboratory: Practical Advice. . . . . . . . . . . . . . . . . . . 1 Anne C. Hart and Heather L. Bennett 2 The Basics of Setting up Successful Teaching Labs and Short-Term Projects with C. elegans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Elizabeth E. Glater 3 Cryopreservation of C. elegans and Other Nematodes with Dimethyl Sulfoxide and Trehalose. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 Kevin F. O’Connell 4 Genetic Methods for Cellular Manipulation in C. elegans . . . . . . . . . . . . . . . . . . . . 51 Menachem Katz 5 Observing and Quantifying Fluorescent Reporters . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Sreeparna Pradhan and Michael Hendricks 6 Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 Amelie Bergs, Thilo Henss, Caspar Glock, Jatin Nagpal, and Alexander Gottschalk 7 Optogenetic Perturbation of Individual C. elegans Pharyngeal Neurons While Monitoring Feeding Behavior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117 Nicholas F. Trojanowski and Christopher Fang-Yen 8 Antibody Staining for Nematodes with Heat-induced Antigen Retrieval (HIAR) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133 Curtis M. Loer 9 ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 Chih-Chieh (Jay) Yu, Danielle M. Orozco Cosio, and Edward S. Boyden 10 A Fusion PCR Method for Expressing Genetic Tools in C. elegans . . . . . . . . . . . . 205 Eduard Bokman, Yifat Eliezer, and Alon Zaslaver 11 Approaches for CRISPR/Cas9 Genome Editing in C. elegans . . . . . . . . . . . . . . . . 215 Charlotte J. Martin and John A. Calarco 12 Targeted and Random Transposon-Assisted Single-Copy Transgene Insertion in C. elegans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 239 Sonia El Mouridi and Christian Frøkjær-Jensen 13 Mutation Mapping and Identification by Whole-Genome Sequencing. . . . . . . . . 257 Harold E. Smith vii14 Lipid Extraction and Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271 Henry H. Harrison and Jennifer L. Watts 15 Isolating Caenorhabditis elegans from the Natural Habitat . . . . . . . . . . . . . . . . . . . 283 Clotilde Gimond, Nausicaa Poullet, and Christian Braendle 16 Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 293 Ross C. Lagoy, Eric Larsen, Dan Lawler, Hamilton White, and Dirk R. Albrecht 17 Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser . . . . . . . . . 319 Maria B. Harreguy, Tracy S. Tran, and Gal Haspel 18 An Imaging System for Monitoring C. elegans Behavior and Aging . . . . . . . . . . . 329 Matthew A. Churgin and Christopher Fang-Yen 19 Methods for Modulating and Measuring Neuromuscular Exertion in C. elegans. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339 Kiley J. Hughes and Andre´s G. Vidal-Gadea 20 Recording and Quantifying C. elegans Behavior . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 Navin Pokala and Steven W. Flavell 21 Primer on Mathematical Modeling in C. elegans . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 Ayush Ranawade and Erel Levine Index . . . . . . . .. . . . . . . . . . . . . . . . . 387 مشخصات فایل |
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| عنوان (Title): | C. elegans_ Methods and Applications |
| نام فایل (File name): | 912-www.GeneProtocols.ir-C. elegans_ Methods and Applications-Humana (2022).pdf |
| عنوان فارسی (Title in Persian): | سینورابدیتیس الگانس- روشها و کاربردها |
| ایجاد کننده: | Gal Haspel (editor), Anne C. Hart (editor) |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 1071621807,9781071621806 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 8.63 مگابایت |
| تعداد صفحات (Book length in pages): | 389 |
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