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فهرست مطالب: Preface . . . . . . . . . . . . . . . vContributors. . . . . . . . . . . ix 1 RNP-Based Control Systems for Genetic Circuits in Synthetic Biology Beyond CRISPR . . . . . . . . 1 Trevor R. Simmons, Andrew D. Ellington, and Lydia M. Contreras 2 Computational Design of RNA Toehold-Mediated Translation Activators . . . . . 33 Kaiyue Wu, Zhaoqing Yan, and Alexander A. Green 3 Design of RNA-Based Translational Repressors . . . . . 49 Seongho Hong, Dongwon Park, Soma Chaudhary, Griffin McCutcheon, Alexander A. Green, and Jongmin Kim 4 Design of Ribocomputing Devices for Complex Cellular Logic . . . 65 Griffin McCutcheon, Soma Chaudhary, Seongho Hong, Dongwon Park, Jongmin Kim, and Alexander A. Green 5 Computational Design of Small Transcription Activating RNAs (STARs) . . . . . . 87 Baiyang Liu and James Chappell 6 Design and Assembly of Multilevel Transcriptional and Translational Regulators for Stringent Control of Gene Expression. . . . . . 99 F. Veronica Greco, Thea Irvine, Claire S. Grierson, and Thomas E. Gorochowski 7 Model-Based Design of Synthetic Antisense RNA for Predictable Gene Repression . . . . . . . . 111 Tae Seok Moon 8 Design of a Toolbox of RNA Thermometers . . . . . . . 125 Shaunak Sen, Abhilash Patel, and Krishan Kumar Gola 9 Development of Synthetic Riboswitches to Guide the Evolution of Metabolite Production in Microorganisms . . . . . . . 135 Minsun Kim, Sungho Jang, and Gyoo Yeol Jung 10 Efficient Method to Identify Synthetic Riboswitches Using RNA-Based Capture-SELEX Combined with In Vivo Screening . . . . . . . 157 Janice Kramat and Beatrix Suess 11 RNA Design Principles for Riboswitches that Regulate RNase P-Mediated tRNA Processing . . . . . . . . 179 Anna Ender, Peter F. Stadler, Mario Mo ¨rl, and Sven Findeiß 12 Design, Characterization, and Application of Targeted Gene Activation in Bacteria Using a Modular CRISPRa System. . . . . . . . 203 Maria Claudia Villegas Kcam and James Chappell 13 Reprogramming TracrRNAs for Multiplexed RNA Detection . . . . 217 Chunlei Jiao and Chase L. Beisel vii14 Harnessing CRISPR-Cas9 for Epigenetic Engineering . . . . 237 Rosa S. Guerra-Resendez and Isaac B. Hilton 15 RNA Structure Prediction, Analysis, and Design: An Introduction to Web-Based Tools . . . . . . . . . . . . . 253 Raphael Angelo I. Zambrano, Carmen Hernandez-Perez, and Melissa K. Takahashi 16 Single-Molecule FRET Studies of RNA Structural Rearrangements and RNA-RNA Interactions. . . . . . . 271 Ewelina M. Małecka, Boyang Hua, and Sarah A. Woodson 17 Cotranscriptional RNA Chemical Probing . . . . . . . . . 291 Courtney E. Szyjka and Eric J. Strobel Index . . . . . . . . . . 331 مشخصات فایل |
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عنوان (Title): | Riboregulator Design and Analysis |
نام فایل (File name): | 928-www.GeneProtocols.ir-Riboregulator Design and Analysis-Humana (2022).pdf |
عنوان فارسی (Title in Persian): | طراحی و تحلیل ریبورگولاتور |
ایجاد کننده: | James Chappell, Melissa K. Takahashi |
زبان (Language): | انگلیسی English |
سال انتشار: | 2022 |
شابک ISBN: | 1071624202, 9781071624203 |
نوع سند (Doc. type): | کتاب |
فرمت (File extention): | |
حجم فایل (File size): | 11.8 مگابایت |
تعداد صفحات (Book length in pages): | 333 |
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برچسب ها:
2022,
انگلیسی,
بیوانفورماتیک,
بیوتکنولوژِی,
مهندسی ژنتیک,
پروتکل,
پروتکل بیوشیمی,
پروتکل ژنتیک,