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قابل دانلود از شنبه, ۱۹ فروردين ۱۴۰۲
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فهرست مطالب: Dedication . . . . . . . . . . . vPreface . . . .. . . . . . . . . . vii Contributors. . . . . . . . . . . . . . . xv PART I MOLECULAR AND CELLULAR ANALYSIS AND MANIPULATION 1 Development of a Multi-target Protein Biomarker Assay for Circulating Tumor Cells . . . . . . . . . . . . . . . 3 2 Method to Isolate Dormant Cancer Cells from Heterogeneous Populations . . . . . . . . . 19 3 Label-Free Morphological Phenotyping of In Vitro 3D Microtumors . . . . . . . . . 31 4 High-Throughput Microenvironment Microarray (MEMA) High-Resolution Imaging. . . . . . . . . 47 5 Real-Time Analysis of AKT Signaling Activities at Single-Cell Resolution Using Cyclic Peptide-Based Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 6 Microfluidic Device Technologies for Digestion, Disaggregation, and Filtration of Tissue Samples for Single Cell Applications . . . . . . . . . . . . . . . . . 81 7 Microdissection Methods Utilizing Single-Cell Subtype Analysis and the Impact on Precision Medicine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 8 Functionalized Lineage Tracing for the Study and Manipulation of Heterogeneous Cell Populations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 9 Fluorescence Lifetime Imaging Probes for Cell-Based Measurements of Enzyme Activity . . . . . . 133 10 Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors . . . . . . . 163 11 Node-Pore Sensing for Characterizing Cells and Extracellular Vesicles. . . . . . . . . 171 12 Affinity-Based Enrichment of Extracellular Vesicles with Lipid Nanoprobes . . . . 185 13 Droplet Magnetofluidic Assay Platform for Quantitative Methylation-Specific PCR. . . . . 199 14 Droplette: A Platform Technology to Directly Deliver Nucleic Acid Therapeutics and Other Molecules into Cells and Deep into Tissue Without Transfection Reagents . . . . . . . . . . 211 15 Molecular Imaging of HER2 in Patient Tissues with Touch Prep-Quantitative Single Molecule Localization Microscopy . . . . . . . . . . . . . . . . . 231 16 Microchip Free-Flow Electrophoresis for Bioanalysis, Sensing, and Purification. . . . . . . . 249 17 Green Chemistry Preservation and Extraction of Biospecimens for Multi-omic Analyses . . . . . . . . 267 18 TdT-dUTP DSB End Labeling (TUDEL), for Specific, Direct In Situ Labeling of DNA Double Strand Breaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . 299 19 Ligand-Directed GPCR Antibody Discovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 319 20 Self-Induced Back-Action Actuated Nanopore Electrophoresis (SANE) Sensor for Label-Free Detection of Cancer Immunotherapy-Relevant Antibody-Ligand Interactions . . . . . . . . . . . . . . . . . . . . 343 21 Incorporating, Quantifying, and Leveraging Noncanonical Amino Acids in Yeast . . . . . . . 377 22 Nuclease-Assisted, Multiplexed Minor-Allele Enrichment: Application in Liquid Biopsy of Cancer . . . . . . 433 23 Implementation of Ion Mobility Spectrometry-Based Separations in Structures for Lossless Ion Manipulations (SLIM) . . . . . . . . . . . . . . . . . . . . . . . . 453 24 Pleural Effusion Aspirate for Use in 3D Lung Cancer Modeling and Chemotherapy Screening . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 471 25 Using Optical Tweezers to Dissect Allosteric Communication Networks in Protein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 485 PART II THERAPEUTICS TECHNOLOGIES 26 Focused Ultrasound-Mediated Intranasal Brain Drug Delivery Technique (FUSIN). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 501 27 Extracellular pH Mapping as Therapeutic Readout of Drug Delivery in Glioblastoma . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 515 28 Charge-Based Multiarm Avidin Nanoconstruct as a Platform Technology for Applications in Drug Delivery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 537 29 Chemical Modification of Proteins and Their Intracellular Delivery Using Lipidoid Nanoparticles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 555 30 Generation of Membrane-Derived Nanovesicles by Nitrogen Cavitation for Drug Targeting Delivery and Immunization . . . . . . . . . . . . . . . . . . 575 31 Laboratory-Scale Production of Sterile Targeted Microbubbles. . . . . . . . . . . . . . . 591 32 Adeno-Associated Viral Vector Immobilization and Local Delivery from Bare Metal Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 601 33 Decellularization and Recellularization Methods for Avian Lungs: An Alternative Approach for Use in Pulmonary Therapeutics. . . . . . . . . . . . . . . . . 617 34 Methods for Forming Human Lymphatic Microvessels In Vitro and Assessing their Drainage Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 651 35 Natural Polymer–Based Micronanostructured Scaffolds for Bone Tissue Engineering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 669 36 Biodegradable Electrospun Nanofibrous Scaffolds for Bone Tissue Engineering. . . . . . . . . . 693 37 Biotribometer for Assessment of Cell and Tissue Toxicity of Orthopedic Metal Implant Debris . . . . . . . . . . . . . . 713 38 Methods for Quantifying Neutrophil Extracellular Traps on Biomaterials . . . . . . . . 727 39 In Vivo Imaging of Implanted Hyaluronic Acid Hydrogel Biodegradation . . . . . . . . . . . . . . . . 743 40 Computational Modeling and Simulation to Quantify the Effects of Obstructions on the Performance of Ventricular Catheters Used in Hydrocephalus Treatment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 767 41 Selection of Cancer Stem Cell–Targeting Agents Using Bacteriophage Display . . . . . . . . . . . . . 787 42 Nanoscintillator-Based X-Ray-Induced Photodynamic Therapy. . . . . . . . . . . . . . . 811 43 Methods to Measure the Inhibition of ABCG2 Transporter and Ferrochelatase Activity to Enhance Aminolevulinic Acid-Protoporphyrin Fluorescence-Guided Tumor Detection and Resection. . . . . . . . . . . . . . . . . . . . 823 Matthew Mansi, Richard Howley, and Bin Chen 44 Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug–Target Engagement. . . . . . . . . . . . . . . . 837 45 A Method of Tumor In Vivo Imaging with a New Peptide-Based Fluorescent Probe . . . . . . . . . . 857 46 Thermal Ablation Treatment for Cervical Precancer (Cervical Intraepithelial Neoplasia Grade 2 or Higher [CIN2+]). . . . . . . . . . . . . . 867 47 Employing Novel Porcine Models of Subcutaneous Pancreatic Cancer to Evaluate Oncological Therapies . . . . . . . . . . . . . . . . . . . . 883 Index . . . . . . . . . . . . 897 مشخصات فایل |
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| عنوان (Title): | Biomedical Engineering Technologies |
| نام فایل (File name): | 901-www.GeneProtocols.ir-Biomedical Engineering Technologies_ Volume 2-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): | فناوری های مهندسی زیست پزشکی |
| ایجاد کننده: | Avraham Rasooly, Houston Baker, Miguel R. Ossandon |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 1071618105, 9781071618103 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 29.7 مگابایت |
| تعداد صفحات (Book length in pages): | 907 |
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