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فهرست مطالب: Preface...................... . . . vContributors..................... . . . . xi 1 Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis...1 Alexa M. Jauregui, Zoe´ M. Cubero Corte´s, Sean D. Meehan, and Sanjoy K. Bhattacharya 2 Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation................ . 7 Blake R. Hopiavuori, Dustin R. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman 3 Multi-macromolecular Extraction from Endosymbiotic Anthozoans... . . . . 17 Anderson B. Mayfield 4 High-Resolution Liquid Chromatography–Mass Spectrometry for Lipidomics.................. . . . . 57 Faith Christine Harvey, Vanessa Collao, and Sanjoy K. Bhattacharya 5 Characterization of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry................ 65 Jennifer Arcuri and Sanjoy K. Bhattacharya 6 Protocol and Methods Applicable to Retinal Vascular Diseases..... . . . 71 Saurabh Kumar, Manjunath B. Joshi, and Inderjeet Kaur 7 Analysis and Annotation of Phospholipids by Mass Spectrometry-Based Metabolomics............. . . . 79 A´ lvaro Gonza´lez-Domı´nguez, Marı´a Santos-Martı´n, Ana Sayago, Alfonso Marı´a Lechuga-Sancho, A´ ngeles Ferna´ndez-Recamales, and Rau´l Gonza´lez-Domı´nguez 8 Profiling the Mammalian Lipidome by Quantitative Shotgun Lipidomics.. 89 Mads Møller Foged, Kenji Maeda, and Mesut Bilgin 9 Lipidomics Analysis with Triple Quadrupole Mass Spectrometry..... . 103 Meher A. Saleem, Anna Mueller, and Sanjoy K. Bhattacharya 10 High-Performance Chromatographic Separation of Cerebrosides.... . 107 Renaud Sicard 11 Quantification of Endocannabinoids from Biological Samples Using Liquid Chromatography–Tandem Mass Spectrometry.....115 Zhiying Wang, Chenglin Mo, Lynda Bonewald, and Marco Brotto 12 Analysis of Lipids, Fatty Acid, and Cholesterol in Membrane Microdomains.................. . . . . 129 Martin-Paul Agbaga, Mark E. McClellan, and Michael H. Elliott 13 Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry..................141 Emily J. Neag, Vanessa Collao, and Sanjoy K. Bhattacharya 14 Analyses and Localization of Phosphatidylcholine and Phosphatidylserine in Murine Ocular Tissue Using Imaging Mass Spectrometry.....149 Varun Krishnan, Sean D. Meehan, Colin Hayter, and Sanjoy K. Bhattacharya 15 A Robust Phenotypic Screening Assay Utilizing Human Podocytes to Identify Agents that Modulate Lipid Droplets........ . . . 163 Margaret Gurumani, Shamroop Kumar Mallela, Javier Varona, Sandra Merscher, Alessia Fornoni, and Hassan Al-Ali 16 Capillary Electrophoresis Plasma Fractionation for Lipids Analysis.... 175 Anddre Osmar Valdivia, Carolyn Cray, and Sanjoy K. Bhattacharya 17 Combined Use of MALDI-TOF Mass Spectrometry and 31P NMR Spectroscopy for the Analysis of (Phospho)Lipids.....183 Jenny Leopold, Kathrin M. Engel, Patricia Prabutzki, and Ju¨rgen Schiller 18 Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles (OMVs) of Pseudomonas Aeruginosa.... 201 Pallavi Lahiri, Priyakshi Gogoi, and Dipankar Ghosh 19 Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells.................. . . 217 Evan B. Stubbs Jr 20 Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate................ 231 Joseph L. Wilkerson, Sandip K. Basu, and Nawajes A. Mandal 21 Lipid Extraction and Sample Preservation Techniques for Stable Isotope Analysis and Ecological Assays............ 241 Kyle H. Elliott, James D. Roth, Kevin Crook, and David Yurkowski 22 Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry..................259 Glenda Vasku, Niyazi Acar, and Olivier Berdeaux 23 Untargeted Analysis of Lipids Containing Very Long Chain Fatty Acids in Retina and Retinal Tight Junctions.......... . . 269 Julia V. Busik, Hannah Decot, Anna B. Lin, and Todd A. Lydic 24 Analysis of Lipid Contents in Human Trabecular Meshwork Cells by Multiple Reaction Monitoring (MRM) Profiling Lipidomics.... . . 291 Ting Wang and Padmanabhan Paranji Pattabiraman 25 Mass Spectrometry Approaches for Detection and Determination of Prostaglandins from Biological Samples.......... . . . 299 Zhiying Wang, Chenglin Mo, Kamal Awad, Lynda Bonewald, and Marco Brotto 26 Downloading and Analysis of Metabolomic and Lipidomic Data from Metabolomics Workbench Using MetaboAnalyst 5.0....... 313 Ashley Howell and Charles Yaros 27 Accurate Analysis of Lipid Building Blocks Using the Tool LipidOne.. . . . 323 Roberto Maria Pellegrino, Matteo Giulietti, Husam B. R. Alabed, Anna Aurora Taddei, Sandra Buratta, Lorena Urbanelli, Francesco Piva, and Carla Emiliani 28 Image-Based Longitudinal Characterization of Corneal Wound to Understand the Role of Sphingosine-1-Phosphate........337 Sandip K. Basu and Nawajes Mandal 29 Characterization of Trabecular Meshwork Mechanical Property Modulation After Application of Lipids............347 Wyndham Batchelor, Jennifer Arcuri, Sanjoy K. Bhattacharya, and Noe ¨l M. Ziebarth 30 C-Laurdan: Membrane Order Visualization of HEK293t Cells by Confocal Microscopy................ . 353 Sean D. Meehan, Colin Hayter, and Sanjoy K. Bhattacharya 31 Sonication-Based Basic Protocol for Liposome Synthesis....... . . 365 Roberto Mendez Index...................... . . . . 371 مشخصات فایل |
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| عنوان (Title): | Lipidomics_ Methods and Protocols |
| نام فایل (File name): | 1006-www.GeneProtocols.ir-Lipidomics_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
لیپیدومیکس - روشها و پروتکل ها
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| ایجاد کننده: | Sanjoy K. Bhattacharya |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071629659 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 11.4 مگابایت |
| تعداد صفحات (Book length in pages): | 375 |
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