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فهرست مطالب: Preface . . . . . . . . . . . . . vContributors . . . . . . . . . ix 1 Construction of Small-Insert and Large-Insert Metagenomic Libraries . . . . . . . . 1 Carola Simon and Rolf Daniel 2 Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies . . . . . . . 13 Jacqueline Hollensteiner, Franziska Wemheuer, Dominik Schneider, Birgit Pfeiffer, and Bernd Wemheuer 3 Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds . . . . . . 23 Nancy Weiland-Brauer € , Livı´a Saleh, and Ruth A. Schmitz 4 Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA . . . . . . . 51 Supathep Tansirichaiya, William Hutton, and Adam P. Roberts 5 Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next-Generation Sequencing Approach . . . . . . 73 Alinne L. R. Santana-Pereira 6 Activity-Based Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes . . . . . . . . 91 Nicole Adam-Beyer and Mirjam Perner 7 Functional Metagenomics Approach for the Discovery of Novel Genes Encoding Phosphatase Activity . . . . . . . . . 103 Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel 8 Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities . . . . . . . . 115 Aranksha Thakor, Jiujun Cheng, and Trevor C. Charles 9 Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures . . . . . . . . . . . 125 Jascha F. H. Macdonald, Ines Krohn, and Wolfgang R. Streit 10 The PET-Degrading Potential of Global Metagenomes: From In Silico Mining to Active Enzymes . . . . . . . . . . . . 139 Jennifer Chow, Pablo Pe´rez-Garcı´a, Robert F. Dierkes, Hongli Zhang, and Wolfgang R. Streit 11 High-Throughput Screening for Thermostable Polyester Hydrolases . . . . . . . . . . 153 Yannick Branson, Christoffel P. S. Badenhorst, Lara Pfaff, Carolin Buchmann, Ren Wei, and Uwe T. Bornscheuer 12 Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System . . . . . . . . . . . . 167 Yuchen Han, Robert F. Dierkes, and Wolfgang R. Streit 13 Assigning Functions of Unknown Enzymes by High-Throughput Enzyme Characterization . . . . . . . 181 Patricia Molina-Espeja, Laura Fernandez-Lopez, Peter N. Golyshin, and Manuel Ferrer 14 Isolation of a Host-Confined Phage Metagenome Allows the Detection of Phages Both Capable and Incapable of Plaque Formation . . . 195 Ines Friedrich and Robert Hertel 15 CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis . . . . . . . . . . . 205 Katharina Kohm, Veronika Theresa Lutz, Ines Friedrich, and Robert Hertel 16 Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production . . . 213 Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Anne´ 17 DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms . . . . . . . . . 261 Eleanor Jameson, Martin Taubert, Roey Angel, Sara Coyotzi, Yin Chen, O¨ zge Eyice, Hendrik Schafer, J. Colin Murrell, Josh D. Neufeld, € and Marc G. Dumont Index . . . . . . . . . . . . . . 283 مشخصات فایل |
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| عنوان (Title): | Metagenomics_ Methods and Protocols |
| نام فایل (File name): | 956-www.GeneProtocols.ir-Metagenomics_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): |
متاژنومیکس - روشها و پروتکل ها
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| ایجاد کننده: | Wolfgang R. Streit, Rolf Daniel |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 1493982745, 9781493982745 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 8.74 مگابایت |
| تعداد صفحات (Book length in pages): | 290 |
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