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فهرست مطالب: Preface . . . . . . . . . . . . . . vContributors . . . . . . . . . . . . . . . xi Contents PART I METHODS FOR THE DETECTION OF MELATONIN AND ITS BIOLOGICAL DERIVATIVES 1 Two-Dimensional Thin Layer Chromatography of Melatonin and Related Compounds . . . . . . . . . 3 David C. Klein 2 Detection of Melatonin in Tissue Samples . . . . . . . . 7 Ce´line Legros and Jean A. Boutin 3 Measuring Dim Light Melatonin Onset in Humans . . . . . 13 Melissa A. St. Hilaire and Steven W. Lockley 4 Measuring Urinary 6-Sulphatoxymelatonin in Humans . . . . . . . 21 Melissa A. St. Hilaire and Steven W. Lockley 5 MEL-Index: Estimation of Tissue Melatonin Levels Using Gene Expression Data . . . . . . . . . . . 29 Pedro Augusto C. M. Fernandes, Regina P. Markus, and Gabriela Sarti Kinker 6 Melatonin Synthesis Enzymes Activity: Radiometric Assays for AANAT, ASMT, and TPH . . . . . . . . . 33 Fernanda Gaspar do Amaral, Jose´ Cipolla-Neto, and Solange Castro Afeche 7 Pinealectomy in Rats . . . . . . . . . . . . 45 Camila Congentino Gallo, Fernanda Akane Nishino, Fernanda Gaspar do Amaral, and Jose´ Cipolla-Neto 8 Surgical Techniques and Nuances for Superior Cervical Ganglionectomy and Decentralization in Rats . . . . . 53 Sarosh Irfan Madhani, David C. Klein, Estela M. Mun˜oz, and Luis E. Savastano 9 Pineal Microdialysis . . . . . . . . . . . . . 63 Caroline Aparecida Pereira de Souza, Fernanda Akane Nishino, Fernanda Gaspar do Amaral, and Jose´ Cipolla-Neto 10 Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland . . . . . . . . . . . . . 75 Martin Fredensborg Rath and Morten Møller 11 Pineal Cells Dissociation and Culture: Isolated Pinealocytes, Isolated Astrocytes, and Co-culture . . . . . . . . . 85 Solange Castro Afeche, Diego de Piazza Pimentel, Luı´s Felipe Ferro, and Jose´ Cipolla-Neto viiviii Contents 12 Pineal Gland Culture . . . . . . . . . . . . 95 Solange Castro Afeche, Fernanda Gaspar do Amaral, and Jose´ Cipolla-Neto 13 Preparation of Single Pineal Cells . . . . . . . . . . . 101 Steven L. Coon and David C. Klein 14 RNA Sequencing of Single Pineal Cells . . . . . . 105 Steven L. Coon and David C. Klein 15 siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells . . . . . . . . . . . 113 Aurea Susana Blancas-Velazquez and Martin Fredensborg Rath 16 Analysis of the Human Pineal Proteome by Mass Spectrometry . . . . . 123 Mariette Matondo, Guillaume Dumas, and Erik Maronde 17 The Pineal Transcriptome Webpage . . . . . . . . . 133 David C. Klein and Steven L. Coon PART II METHODS TO MEASURE MELATONIN EFFECTS AT THE MOLECULAR AND CELLULAR LEVEL 18 2-[125I]iodomelatonin and [3H]melatonin Binding Assays for Melatonin Receptors . . . . . . . . . 141 Florence Gbahou and Ralf Jockers 19 Alternative Ligands at Melatonin Receptors . . . . . . . 151 Ce´line Legros, Said Yous, and Jean A. Boutin 20 GTPγS Binding Assay for Melatonin Receptors in Mouse Brain Tissue . . . 163 Romain Gerbier and Ralf Jockers 21 MT1 Melatonin Receptor Reconstitution in Nanodiscs . . . . . . . 171 Jean A. Boutin, Christel Logez, Marjorie Damian, Renaud Wagner, Jean-Louis Bane`res, and Gilles Ferry 22 Functional Investigation of Melatonin Receptor Activation by Homogenous cAMP Assay . . . . . . . . . 179 Erika Cecon, Jean-Luc Guillaume, and Ralf Jockers 23 Functionality of Melatonin Receptors: Internalization . . . . . . . . 189 Cle´mence Dupre´, Ce´line Legros, and Jean A. Boutin 24 Functionality of Melatonin Receptors: Recruitment of β-Arrestin at MT1 . 195 Cle´mence Dupre´, Ce´line Legros, and Jean A. Boutin 25 MT1 Receptor Signaling Pathways by Impedance Measurement . . . . 201 Anne Bonnaud, Cle´mence Dupre´, Ce´line Legros, and Jean A. Boutin 26 Measuring Protein-Protein Interactions of Melatonin Receptors by Bioluminescence Resonance Energy Transfer (BRET) . . . . . . 207 Atsuro Oishi and Ralf Jockers 27 Nonradioactive In Situ Hybridization of MT1 Melatonin Receptor for the Identification of Melatonin Target Cells . . . . . 219 Paul KlosenContents ix 28 Beta-Galactosidase as a Transgenic Reporter for the Mapping and Phenotyping of MT1 and MT2 Melatonin Receptor-Expressing Cells . 243 Paul Klosen 29 Why Are We Still Cloning Melatonin Receptors? A Commentary . . . 267 Ce´lia Gautier, Isabelle Theret, Giulia Lizzo, Gilles Ferry, Sophie-Pe´ne´lope Gue´nin, and Jean A. Boutin 30 Measuring Binding at the Putative Melatonin Receptor MT3 . . . . . . . 283 Ce´line Legros, Philippe Dupuis, Gilles Ferry, and Jean A. Boutin 31 Cloning, Expression, Purification, Crystallization, and X-Ray Structural Determination of the Human NQO2 in Complex with Melatonin . . . . . . . . . . . . 291 Barbara Calamini, Gilles Ferry, and Jean A. Boutin 32 Melatonin Binding to Human NQO2 by Isothermal Titration Calorimetry . . . . . . . . . . 305 Barbara Calamini, Gilles Ferry, and Jean A. Boutin 33 Measurement of NQO2 Catalytic Activity and of Its Inhibition by Melatonin . . . . . . . . . . . . . . 315 Gilles Ferry and Jean A. Boutin 34 Measuring the NQO2: Melatonin Complex by Native Nano-Electrospray Ionization Mass Spectrometry . . . . . . 323 Jean A. Boutin, Johann Stojko, Gilles Ferry, and Sarah Cianferani 35 Human and Rodent Cell Lines as Models of Functional Melatonin-Responsive Pancreatic Islet Cells . . . . . . . 329 Juliane Zibolka, Ina Bahr, Elmar Peschke, Eckhard Mu € ¨hlbauer, and Ivonne Bazwinsky-Wutschke 36 Mesenchymal Stem Cell and Monocyte Co-cultures . . . . . 353 Fahima Munmun and Paula A. Witt-Enderby PART III METHODS TO MEASURE MELATONIN EFFECTS IN VIVO 37 Real-Time Monitoring of Circadian Rhythms in the Eye . . . . . . 367 Kenkichi Baba and Gianluca Tosini 38 Assessing the Role of Melatonin in the Modulation of Visual Functions in the Mouse . . . . . . . . . 377 Kenkichi Baba and Gianluca Tosini 39 Methods to Assess Melatonin Receptor-Mediated Phase-Shift and Re-entrainment of Rhythmic Behaviors in Mouse Models . . . . . . 391 Grant C. Glatfelter, Jennifer Sosa, Randall L. Hudson, and Margarita L. Dubocovich 40 Neurotransmission Recovery by Melatonin Measured by CMAP . . . . 413 Samuele Negro, Marco Stazi, Michela Rigoni, and Aram Megighian 41 Olfaction and Melatonin: The Use of the Olfactory Discrimination Test . . 425 Ana Carolina Duarte Noseda and Marcelo M. S. Limax Contents 42 Photothrombotic Mouse Models for the Study of Melatonin as a Therapeutic Tool After Ischemic Stroke . . . . . . . 433 Marco Cambiaghi, Laura Cherchi, and Stefano Comai 43 Hypnotic Effects of Melatonergic Compounds Measured in Mice or Rats . . . . . . . . . . . . 443 Stefano Comai and Danilo De Gregorio 44 Acute and Chronic Pain Preclinical Models to Study the Analgesic Properties of Melatonergic Compounds . . . . . . . . 453 Danilo De Gregorio and Stefano Comai 45 Behavioral and Fluorescent-Based Immunohistochemistry Protocols for Examining Antidepressant-Like Effects of Melatonin in Mice . . . . . . . . . . . . . . 463 Estrada-Reyes Rosa, Mun˜oz Estrada Jesu´ s, and Benı´tez-King Gloria 46 A Method for Perfusion of Tissue-Isolated Human Tumor Xenografts in Nude Rats to Investigate the Oncostatic Role of the Physiological Nocturnal Melatonin Signal . . . . . . . . . . . 477 Robert T. Dauchy, Steven M. Hill, and David E. Blask 47 A Method for Growing Tissue-Isolated Human Tumor Xenografts in Nude Rats for Melatonin/Cancer Studies . . . . . . 489 Robert T. Dauchy, Steven M. Hill, and David E. Blask Index . . . . . . . . . . . . . . . 497 مشخصات فایل |
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| عنوان (Title): | Melatonin_ Methods and Protocols |
| نام فایل (File name): | 954-www.GeneProtocols.ir-Melatonin_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): |
ملاتونین - روشها و پروتکل ها
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| ایجاد کننده: | Ralf Jockers, Erika Cecon |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 1071625926, 9781071625927 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 15.8 مگابایت |
| تعداد صفحات (Book length in pages): | 492 |
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