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فهرست مطالب: Preface ............ . . vContributors........... . . . . xi 1 Cartilage Tissue Engineering: An Introduction ..... . . . . 1 Martin J. Stoddart, Elena Della Bella, and Angela R. Armiento 2 Chondrocyte Isolation and Expansion ....... 9 Paul Harrison, Timothy Hopkins, Charlotte Hulme, Helen McCarthy, and Karina Wright 3 Isolation of Chondrons from Hyaline Cartilage..... . . . . 21 Jasmijn V. Korpershoek, Margot Rikkers, and Lucienne A. Vonk 4 Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation ........ . . 29 Ilyas M. Khan, Joshua McKenna, and Yadan Zhang 5 Physioxic Culture of Chondrogenic Cells ...... . . . 45 Girish Pattappa, Brandon D. Markway, Denitsa Docheva, and Brian Johnstone 6 Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells...... 65 Angela R. Armiento, Yann D. Ladner, Elena Della Bella, and Martin J. Stoddart 7 Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation . . . . . 75 Justyna M. Buchert, Benedict Lotz, Solvig Diederichs, and Wiltrud Richter 8 Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells ........... 87 Amanda R. Dicks, Nancy Steward, Farshid Guilak, and Chia-Lung Wu 9 The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans........ 115 Yann D. Ladner, Mauro Alini, and Angela R. Armiento 10 Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy ...123 Alexander Otahal, Andrea De Luna, Ali Mobasheri, and Stefan Nehrer 11 Metabolomic Profiling to Understand Chondrocyte Metabolism ... 141 Priyanka P. Brahmachary, Hope D. Welhaven, and Ronald K. June vii viii Contents 12 Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes .......... . 157 Huan Meng, Clare L. Thompson, Clarissa R. Coveney, Angus K. Wann, and Martin M. Knight 13 Co-culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads .......... 177 Teresa Z. Brose, Yann D. Ladner, E. Johanna Kubosch, Martin J. Stoddart, and Angela R. Armiento 14 Isolation of Murine Articular Chondrocytes for Single-Cell RNA or Bulk RNA Sequencing Analysis ....... . . . 187 Jillian L. McCool, Nicholas R. Hum, Aimy Sebastian, and Gabriela G. Loots 15 Modulation of MicroRNA Expression During In Vitro Chondrogenesis.. 197 Austin Bell-Hensley, Hongjun Zheng, and Audrey McAlinden 16 Purification and Isolation of Proteins from Hyaline Cartilage... . . . . 217 Makenna J. Hardy, Xinzhu Pu, and Julia Thom Oxford 17 Histological Preparation and Evaluation of Cartilage Specimens ... . 227 Salim E. Darwiche, Milena Tegelkamp, Katja Nuss, and Brigitte von Rechenberg 18 Articular Cartilage Imaging in the Context of the Superficial Chondrocyte Spatial Organization (SCSO) as a Surrogate Marker for Functional Pathology ........265 Bodo Kurz, Thomas Lange, Marita Voelker, and Bernd Rolauffs 19 Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization ........ 271 Seyed Ali Elahi, Rocı´o Castro-Vin˜uelas, Anke Govaerts, Rik Lories, Nele Famaey, and Ilse Jonkers 20 Viral Gene Delivery in Chondrocytes....... . 289 Christopher V. Nagelli, Christopher H. Evans, and Rodolfo E. De La Vega 21 Decellularization of Articular Cartilage: A Hydrochloric Acid– Based Strategy.......... . . . 301 Cornelia Schneider and Sylvia Nu¨rnberger 22 Self-Assembly Culture Model for Engineering Musculoskeletal Tissues .. . 313 Nicholas N. DePhillipo, Jerahme Martinez, and George R. Dodge 23 Osteochondral Explant Isolation and Culture Under a Compression and Shear Bioreactor ......... . . . 325 M. Letizia Vainieri and Sibylle Grad 24 A Mouse Model of Acute Cartilage Injury and Repair .... . . . . 337 Anne-Sophie Thorup, Francesco Dell’Accio, and Suzanne E. Eldridge Contents ix 25 Small-Animal Compression Models of Osteoarthritis....345 Blaine A. Christiansen, Deva D. Chan, Marjolein C. H. van der Meulen, and Tristan Maerz 26 Modeling and Assessing Osteoarthritis in Mice by Destabilization of the Medial Meniscus (DMM) .......357 Jadwiga Miotla-Zarebska, Ida Parisi, Vicky Batchelor, Tonia L. Vincent, and Paul K. Potter 27 Collagenase-Induced Osteoarthritis in Mice...... . 375 Peter M. van der Kraan 28 Platelet-Rich Plasma for Intra-articular Injections: Preclinical and Clinical Evidence......... . . . 381 Angelo Boffa and Giuseppe Filardo Index ............ . . . 391 مشخصات فایل |
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| عنوان (Title): | Cartilage Tissue Engineering |
| نام فایل (File name): | 989-www.GeneProtocols.ir-Cartilage Tissue Engineering-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
مهندسی بافت غضروف
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| ایجاد کننده: | Martin J. Stoddart, Elena Della Bella, Angela R. Armiento |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071628386 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 15.5 مگابایت |
| تعداد صفحات (Book length in pages): | 392 |
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