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فهرست مطالب: Preface . . . . . . . . . . . . . . . vContributors . . . . . . . . . . . ix 1 Purification of MAO A and MAO B from Mammalian Tissue Sources . . . 1 Dale E. Edmondson 2 Purification of Recombinant Eukaryotic MAO A and MAO B Utilizing the Pichia pastoris Expression System . . . . . 11 Dale E. Edmondson 3 The Peroxidase-Coupled Assay to Measure MAO Enzymatic Activity . . . . 23 Joana Reis and Claudia Binda 4 Measurement of MAO Enzymatic Activity by Spectrophotometric Direct Assays . . . . . . . . . . . 35 Joana Reis and Claudia Binda 5 Radiochemical Assay of Monoamine Oxidase Activity . . . . . 45 Andrew Holt 6 MAO Visible Spectroscopy for Ligand Interactions, Redox Chemistry, and Kinetics of Irreversible Inhibition . . . . . . . 63 Rona R. Ramsay 7 Conventional Receptor Radioligand Binding Techniques Applied to the Study of Monoamine Oxidase . . . . . . . . 75 Andrew Holt 8 Assay of MAO Inhibition by Chromatographic Techniques (HPLC/HPLC-MS) . . . . . . . . . . 97 Toma´s Herraiz 9 Crystallization of Human Monoamine Oxidase B . . . . . . . . . 115 Claudia Binda, Dale E. Edmondson, and Andrea Mattevi 10 Detecting Monoamine Oxidase A and B Proteins: A Western Blotting Protocol and Some Practical Considerations . . . . . . . 123 Jennifer N. K. Nyarko, Ryan M. Heistad, Paul R. Pennington, and Darrell D. Mousseau 11 An (Immuno) Fluorescence Protocol for Monitoring Monoamine Oxidase A/B Protein Distribution Within the Cell . . . . . . . . 143 Tyler J. Wenzel, Jennifer N. K. Nyarko, Ryan M. Heistad, Paul R. Pennington, Chris P. Phenix, and Darrell D. Mousseau 12 Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene Transfer . . . . . . 163 Yohan Santin, Angelo Parini, and Jeanne Mialet-Perez 13 In Vitro and In Vivo Assays Characterizing MAO A Function in Cancers . . . . . . 171 Boyang Jason Wu and Jean C. Shih 14 In Vivo Study of Monoamine Oxidases Using Multisite Intracerebral Microdialysis . . . . . . . . . . . 183 Philippe De Deurwaerde`re, Nouhad Samb, Hasna El Boukhari, Re´mi Corne, Abdeslam Chagraoui, and Giuseppe Di Giovanni 15 Informed Use of 3D-QSAR for the Rational Design of Coumarin Derivatives as Potent and Selective MAO B Inhibitors . . . . . 197 Nicola Gambacorta, Marco Catto, Leonardo Pisani, Angelo Carotti, and Orazio Nicolotti 16 Hansch-Type QSAR Models for the Rational Design of MAO Inhibitors: Basic Principles and Methodology . . . . . . 207 Leonardo Pisani, Modesto de Candia, Mariagrazia Rullo, and Cosimo D. Altomare 17 Computational Chemistry and Molecular Modeling of Reversible MAO Inhibitors . . . . . . . . 221 Kemal Yelekc¸i and Safiye Sag˘ Erdem Index . . . . . . . . . . 253 مشخصات فایل |
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| عنوان (Title): | Monoamine Oxidase_ Methods and Protocols |
| نام فایل (File name): | 959-www.GeneProtocols.ir-Monoamine Oxidase_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): |
مونو آمین اکسیداز - روشها و پروتکل ها
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| ایجاد کننده: | Claudia Binda |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 1071626426, 9781071626429 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 9.23 مگابایت |
| تعداد صفحات (Book length in pages): | 259 |
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