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فهرست مطالب: Preface ............ . . vContributors........... . . . . ix PART I CELLULAR ANALYSES OF NEURAL PROGENITORS 1 Dissociation of Cerebellar Granule Neuron Progenitors for Culture, FACS, Transcriptomics, and Molecular Biology..... . . . . 3 Jennifer Karin Ocasio 2 Maintaining Cerebellar Granule Neuron Progenitors in Cell Culture .. . . . 9 Jennifer Karin Ocasio 3 Proliferation Analysis of Cerebellar Granule Neuron Progenitors for Microcephaly Research, Using Immunofluorescent Staining and Flow Cytometry ......... . . . 13 Jennifer Karin Ocasio 4 Conventional and Spectral Karyotyping of Murine Cerebellar Granule Neuron Progenitors ........ . . 25 Gouri Nanjangud PART II ANALYSES OF NEURAL PROGENITORS IN SITU 5 Processing Neonatal Mouse Brains for Immunohistochemical Analysis: As Required for Including Spindle Orientation Analysis and Analysis of DNA Damage and Apoptosis .......49 Gabriela De la Cruz, Nana Nikolaishvili-Feinberg, Scott E. Williams, and Timothy R. Gershon 6 Automated Immunofluorescence Staining for Analysis of DNA Damage and Apoptosis in Brain Sections........ 55 Gabriela De la Cruz, Nana Nikolaishvili-Feinberg, and Timothy R. Gershon 7 Automated Immunofluorescence Staining for Analysis of Mitotic Stages and Division Orientation in Brain Sections ...... . . 63 Gabriela De la Cruz, Nana Nikolaishvili Feinberg, and Scott E. Williams PART III RNA-SEQ ANALYSIS WITH SINGLE-CELL RESOLUTION (SCRNA-SEQ) 8 scRNA-seq for Microcephaly Research [I]: Single-Cell Droplet Encapsulation, mRNA Capture, and cDNA Synthesis .... . . . . 83 Benjamin Babcock and Seth Weir 9 scRNA-seq for Microcephaly Research [II]: Preparation of Single-Cell cDNA Libraries.......... . . 99 Benjamin Babcock and Seth Weir 10 scRNA-seq for Microcephaly Research [III]: Computational Analysis of scRNA-seq Data.........105 Benjamin Babcock and Daniel Malawsky 11 scRNA-seq for Microcephaly Research [IV]: Dirichlet Regression for Single-Cell Population Differences....... 123 Daniel Malawsky and Timothy R. Gershon PART IV MRI ANALYSES OF THE MOUSE BRAIN 12 Mouse Brain MRI: Including In Vivo, Ex Vivo, and fcMRI for the Study of Microcephaly ........ . 129 Martin J. MacKinnon, Tzu-Wen W. Wang, and Yen-Yu I. Shih 13 Whole-Body Mouse Fluxomic Analysis to Detect Metabolic Disruptions Associated with Microcephaly: Using 13C Isotopes ... . 149 Andrey P. Tikunov PART V BEHAVIORAL PHENOTYPING IN NEONATAL MICE 14 Neonatal Behavioral Screen for Mouse Models of Neurodevelopmental Disorders ........... . 159 Kathryn M. Harper, Viktoriya D. Nikolova, Monika E. Conrad, and Sheryl S. Moy Index ............ . . . 175 مشخصات فایل |
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| عنوان (Title): | Microcephaly_ Methods and Protocols |
| نام فایل (File name): | 981-www.GeneProtocols.ir-Microcephaly_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
میکروسفالی _ روش ها و پروتکل ها
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| ایجاد کننده: | Timothy Gershon |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071627518, 9781071627525 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 8.25 مگابایت |
| تعداد صفحات (Book length in pages): | 173 |
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