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فهرست مطالب: Preface . . . . . . . . . . . . . vContributors . . . . . . . . . ix Contents PART I GENETIC STRATEGIES TO STUDY ESSENTIAL LPS BIOGENESIS GENES 1 Use of Mutagenesis and Functional Screens to Characterize Essential Genes Involved in Lipopolysaccharide Transport . . . . . . . 3 Andrew Wilson, Carlos Iniguez, and Natividad Ruiz 2 Generation of Stable and Unmarked Conditional Mutants in Pseudomonas aeruginosa . . . . . . . . . . . . 21 Alessandra Lo Sciuto, Maria Concetta Spinnato, Martina Pasqua, and Francesco Imperi 3 Analyzing the Function of Essential Genes by Plasmid Shuffling . . . . . . . 37 Federica Anna Falchi PART II FUNCTIONAL ANALYSIS OF PROTEINS INVOLVED IN LPS TRANSPORT 4 Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia coli . . . . . . . 53 Elisabete C. Cardoso Mendes Moura, Alessandra Polissi, and Paola Sperandeo 5 Exploring the Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in Silico and Biochemical Analyses . . . . . . 71 Julia Monjara´s Feria and Miguel A. Valvano 6 Use of Site-Directed Spin Labeling EPR Spectroscopy to Study Protein–LPS Interactions . . . . . . 83 Kathryn M. Schultz and Candice S. Klug 7 A Photo-Crosslinking Approach to Monitoring the Assembly of an LptD Intermediate with LptE in a Living Cell . . . . 97 Ryoji Miyazaki, Hiroyuki Mori, and Yoshinori Akiyama 8 Mass Spectrometry Analysis of Dynamics and Interactions of the LPS Translocon LptDE . . . . . . . . . . . . 109 Francesco Fiorentino and Jani R. Bolla 9 Affinity Purification and Coimmunoprecipitation of Transenvelope Protein Complexes in Gram-Negative Bacteria . . . . . . . . . 129 Alessandra M. Martorana, Carlo Santambrogio, and Alessandra Polissi 10 A Bacterial Two-Hybrid System for In Vivo Assays of Protein-Protein Interactions and Drug Discovery . . . . . . 145 Daniel Ladant 11 Detecting Lipoproteins Sneaking Out of the Lipopolysaccharide Leaflet. . . . . . . 169 Naemi Csoma, Didier Colau, and Jean-Franc¸ois Collet vii viii Contents PART III TOOLS FOR STRUCTURAL ANALYSIS OF LPS AND LPS TRANSPORT PROTEINS 12 Dissecting Lipopolysaccharide Composition and Structure by GC-MS and MALDI Spectrometry . . . . . . . . . . . . 181 Pilar Garcia-Vello, Immacolata Speciale, Flaviana Di Lorenzo, Antonio Molinaro, and Cristina De Castro 13 Probing the Functional Interaction Interface of Lipopolysaccharide and Antimicrobial Peptides: A Solution-State NMR Perspective . . . . . . . 211 Karishma Biswas and Anirban Bhunia 14 Cryo-EM Analysis of the Lipopolysaccharide Flippase MsbA . . . 233 Franc¸ois A. The´lot and Maofu Liao 15 Protein Crystallization of Two Recombinant Lpt Proteins . . . . . 249 Michela Bollati and Louise J. Gourlay PART IV MICROSCOPY-BASED APPROACHES 16 Metabolic Incorporation of Azido-Sugars into LPS to Enable Live-Cell Fluorescence Imaging . . . . . . . . . 267 Inga Nilsson and David A. Six 17 Use of Atomic Force Microscopy to Characterize LPS Perturbations . . . 279 Yoo Jin Oh Index . . . . . . . . . . . . . . 289 مشخصات فایل |
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| عنوان (Title): | Lipopolysaccharide Transport_ Methods and Protocols |
| نام فایل (File name): | 946-www.GeneProtocols.ir-Lipopolysaccharide Transport_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): | نقل و انتقال لیپوپلی ساکاریدها - روشها و پروتکل ها |
| ایجاد کننده: | Paola Sperandeo |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 1071625802, 9781071625804 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 10.4 مگابایت |
| تعداد صفحات (Book length in pages): | 288 |
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