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فهرست مطالب: Preface...................vContributors................... . xi Contents 1 Histochemistry in Advanced Cytometry: From Fluorochromes to Mass Probes................ . 1 Giuliano Mazzini and Marco Danova PART I VITAL HISTOCHEMISTRY 2 Autofluorescence Label-Free Imaging of the Liver Reticular Structure... . 29 Anna C. Croce, Giuseppina Palladini, Andrea Ferrigno, and Mariapia Vairetti 3 Lysosome Imaging Based on Fluorescent Carbon Dots....... 37 Shuo Guo, Yuanqiang Sun, and Zhaohui Li 4 Oxidative and Nitrosative Stress Detection in Human Sperm Using Fluorescent Probes.............45 Sara Escada-Rebelo and Joa˜o Ramalho-Santos 5 Simultaneous Labeling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis.......... . . 53 Patrizia Vaghi, Amanda Oldani, Paola Fulghieri, Lidia Pollara, Enza Maria Valente, and Virginie Sottile PART II CARBOHYDRATE HISTOCHEMISTRY 6 Lectin Histochemistry: Historical Perspectives, State of the Art, and Future Directions.............. . . 65 Susan Ann Brooks 7 Histochemical and Immunohistochemical Methods for the Identification of Proteoglycans............ . 85 David Sa´nchez-Porras, Juan Varas, Carlos Godoy-Guzma´n, Fabiola Bermejo-Casares, Sebastia´n San Martı´n, and Vı´ctor Carriel 8 Combined Lectin- and Immuno-histochemistry (CLIH) for Fluorescence Microscopy.............99 Das ˇa Zupancˇicˇ, Mateja Erdani Kreft, and Rok Romih PART III PROTEIN HISTOCHEMISTRY 9 Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease............ . . . . . 113 Marie E. Esper, Kasun Kodippili, and Michael A. Rudnicki vii viii Contents 10 Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue... . 133 Sabina Berezowska and Jose´ A. Galva´ n 11 Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin-Fixed and Paraffin-Embedded Tissues............ . . . . 141 Tereza Losmanova´, Mario P. Tschan, Jose´ A. Galva´n, and Sabina Berezowska 12 Immunogold Labeling of Milk Proteins at Transmission Electron Microscopy.............. . . . 149 Paolo D’Incecco 13 Rediscover Potassium Permanganate as a Stain for Basic Proteins on Ultrathin Sections at Transmission Electron Microscopy..... . . . 159 Lorena Zannino, Claudio Casali, and Marco Biggiogera PART IV LIPID HISTOCHEMISTRY 14 Tissue Fixation and Processing for the Histological Identification of Lipids.................. 175 David Sa´ nchez-Porras, Fabiola Bermejo-Casares, Ramon Carmona, Tamara Weiss, Fernando Campos, and Vı´ctor Carriel 15 Staining Methods for Normal and Regenerative Myelin in the Nervous System................ 187 O´ scar D. Garcı´a-Garcı´a, Tamara Weiss, Jesu´ s Chato-Astrain, Stefania Raimondo, and Vı´ctor Carriel 16 Nile Red and BODIPY Staining of Lipid Droplets in Mouse Oocytes and Embryos.............. . . 205 Simona Bisogno, Łukasz Ga˛sior, and Graz˙yna E. Ptak PART V NUCLEAR HISTOCHEMISTRY 17 Chromatin Dispersion Test to Asses DNA Damage in Cervical Epithelial Cells................ . 215 Elva I. Corte´s-Gutie´rrez, Jose´ L. Ferna´ndez, Martha I. Da´ vila-Rodrı´guez, Carlos Garcı´a de la Vega, and Jaime Gosa´ lvez 18 Uranyl-Free Staining as a Suitable Contrasting Technique for Nuclear Structures at Transmission Electron Microscopy.....225 Maria Assunta Lacavalla and Barbara Cisterna 19 Specific RNA Visualization at Electron Microscopy via Terbium Citrate Vapors................ . . 233 Claudio Casali, Lorena Zannino, and Marco Biggiogera PART VI PLANT HISTOCHEMISTRY 20 Localizing Molecules in Plant Cell Walls Using Fluorescence Microscopy. . . . . . 243 Lloyd A. Donaldson Contents ix 21 Ratiometric Fluorescent Safranin-O Staining Allows the Quantification of Lignin Contents In Muro........ . . . . 261 Oriane Morel, Corentin Spriet, Ce´dric Lion, Fabien Baldacci-Cresp, Garance Pontier, Marie Baucher, Christophe Biot, Simon Hawkins, and Godfrey Neutelings 22 Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls................. 269 Youssef Chebli and Anja Geitmann 23 Staining Starch with Iodine Solution.......... . . . 281 Shengnan Zhao, Yinhui Ren, and Cunxu Wei 24 Histochemical Analysis of Plant Secretory Structures...... . . . 291 Diego Demarco PART VII HISTOCHEMISTRY FOR NANOSCIENCE 25 Alcian Blue Staining to Visualize Intracellular Hyaluronic Acid-Based Nanoparticles.............313 Mathieu Repellin, Flavia Carton, Giovanna Lollo, and Manuela Malatesta 26 Prussian Blue Staining to Visualize Iron Oxide Nanoparticles.... . . 321 Valeria Bitonto, Francesca Garello, Arnaud Scherberich, and Miriam Filippi 27 Diaminobenzidine Photooxidation to Visualize Fluorescent Nanoparticles in Adhering Cultured Cells at Transmission Electron Microscopy.............. . . . 333 Manuela Costanzo and Manuela Malatesta 28 Fluorescent Labeling of Lignin Nanocapsules with Fluorol Yellow 088................. . . . . 345 Franco Cheli, Sara Falsini, Maria Cristina Salvatici, Sandra Ristori, Silvia Schiff, Emilio Corti, Irene Costantini, Cristina Gonnelli, Francesco Saverio Pavone, and Alessio Papini Index.................... 355 مشخصات فایل |
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| عنوان (Title): | Histochemistry of Single Molecules_ Methods and Protocols |
| نام فایل (File name): | 966-www.GeneProtocols.ir-Histochemistry of Single Molecules_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
هیستوشیمی سلولهای منفرد - روشها و پروتکل ها
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| ایجاد کننده: | Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071626740 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 19 مگابایت |
| تعداد صفحات (Book length in pages): | 351 |
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