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قابل دانلود از شنبه, ۱۹ فروردين ۱۴۰۲
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فهرست مطالب: Preface . . . . . . . vContributors. . . . . . . . . . . ix PART I DETERMINATION OF UNFOLDED PROTEIN LEVELS 1 Measuring Cysteine Exposure in Unfolded Proteins with Tetraphenylethene Maleimide and its Analogs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2 Fluorescence-Based Biosensors for the Detection of the Unfolded Protein Response . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 3 Indirect Methods To Measure Unfolded Proteins In Living Cells Using Fluorescent Proteins. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 4 A Platform Technology for Monitoring the Unfolded Protein Response . . . . . . . 45 5 The Use of Fluorescent Protein Fusions to Monitor the Unfolded Protein Response and Protein Foldase-Substrate Interactions in Plant Protoplasts . . . . . . . 69 PART II METHODS TO STUDY UPR SIGNAL TRANSMISSION 6 High-Throughput Analysis of Protein Turnover with Tandem Fluorescent Protein Timers. . . . . . . . . . . . 85 7 Detection of HAC1 mRNA Splicing by RT-PCR in Saccharomyces cerevisiae. . . . . . . . . . 101 PART III ANALYSING THE OUTCOMES OF THE UPR PATHWAY ACTIVATION 8 Detecting the Non-conventional mRNA Splicing and Translational Activation of HAC1 in Budding Yeast. . . . . 113 9 Determination of the Stability and Intracellular (Intra-Nuclear) Targeting and Recruitment of Pre-HAC1 mRNA in the Saccharomyces cerevisiae During the Activation of UPR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 10 Assays to Study IRE1 Activation and Signaling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 11 Analysis of XBP1 Contribution to Hyperosmolarity-Induced Lipid Synthesis . . . . . . . . . 169 12 Integrating an ER Stress Reporter for Monitoring Genome-Wide UPR-ER in Budding Yeast . . . . . . . . . 189 PART IV UPR STUDIES IN MAMMALIAN MODELS 13 Analyze Mouse Knockout Models of UPR Pathway Elements . . . . . . . . . . . . . . . . 205 14 In Vitro Stimulation of IRE1α/XBP1-Deficient B Cells with LPS . . . . . . . . . . . . 221 15 Detection of PERK Signaling in the Central Nervous System . . . . . . . . . . . . . . . . 233 PART V UPR IN ALTERNATIVE MODELS 16 Methods to Study the Mitochondrial Unfolded Protein Response (UPRmt) in Caenorhabditis elegans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 249 17 Drosophila Unfolded Protein Response (UPR) Assays In Vitro and In Vivo . . . . . . . 261 18 Protein Preparation for Proteomic Analysis of the Unfolded Protein Response in Arabidopsis thaliana . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 PART VI UPR AND DISEASE 19 Structure-Based Drug Discovery of IRE1 Modulators. . . . . . . . . . . . . . . . . . . . . . . 293 20 HTS Identification of Activators and Inhibitors of Endoplasmic Reticulum (ER) Stress and the Unfolded Protein Response (UPR) . . . . . . . . . . . . . . . . . . . . . . . . . . 317 Index . . . . . . . . . 329 مشخصات فایل |
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| عنوان (Title): | The Unfolded Protein Response_ Methods and Protocols |
| نام فایل (File name): | 900-www.GeneProtocols.ir-The Unfolded Protein Response_ Methods and Protocols-Humana (2022).pdf |
| عنوان فارسی (Title in Persian): | پاسخ پروتئین آشکار شده - روشها و پروتکل ها |
| ایجاد کننده: | Roberto Pérez-Torrado |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 1071617311, 9781071617311 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 10 مگابایت |
| تعداد صفحات (Book length in pages): | 326 |
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