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قابل دانلود از سه شنبه, ۲۶ ارديبهشت ۱۴۰۲
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فهرست مطالب: Preface “Plant Proteostasis”....... . . . vContributors.......... . xiii PART I UBIQUITIN CONJUGATION AND DECONJUGATION ANALYSIS 1 Observing Real-Time Ubiquitination in High Throughput with Fluorescence Polarization...... . 3 Tyler G. Franklin and Jonathan N. Pruneda 2 Identification and Characterization of Physiological Pairing of E2 Ubiquitin-Conjugating Enzymes and E3 Ubiquitin Ligases.. . . . . 13 Carla Brillada and Marco Trujillo 3 Immunoprecipitation of Cullin–Ring Ligases (CRLs) in Arabidopsis thaliana Seedlings....... . . . . . 31 Federica Casagrande and Giovanna Serino 4 An In vitro Assay to Recapitulate Hormone-Triggered and SCF-Mediated Protein Ubiquitylation.... . . . . . 43 Michael Niemeyer, Jhonny Oscar Figueroa Parra, and Luz Irina A. Calderon Villalobos 5 Analysis of Proteasome-Associated Ubiquitin Ligase Activity.. . . . 57 Zhishuo Wang, Beatriz Orosa-Puente, and Steven H. Spoel 6 Measuring the DUB Activity of Arabidopsis Deubiquitylating Enzymes...... . . . . . 69 Karin Vogel, Marie-Kristin Nagel, and Erika Isono PART II SUMO CONJUGATION AND DECONJUGATION 7 SUMO Conjugation and SUMO Chain Formation by Plant Enzymes....... . . . . . . 83 Konstantin Tomanov, Jose Julian, Ionida Ziba, and Andreas Bachmair 8 Kinetic Analysis of Plant SUMO Conjugation Machinery.. . . . . . . 93 Laura Castan˜o-Miquel and L. Maria Lois 9 Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases....... . . . . . . 109 Prakash Kumar Bhagat, Dipan Roy, and Ari Sadanandom PART III ANALYSIS OF AUTOPHAGY 10 Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii...... . . . . 123 Jose´ L. Crespo and Marı´a Esther Pe´rez-Pe´rez ix x Contents 11 Detection of Autophagy in Plants by Fluorescence Microscopy.. . 135 Yunting Pu and Diane C. Bassham 12 Characterization of ATG8-Family Interactors by Isothermal Titration Calorimetry....... . . 149 Lorenzo Picchianti, Arthur Sedivy, and Yasin Dagdas PART IV PROTEIN TURNOVER AND STABILITY 13 Protocols for Studying Protein Stability in an Arabidopsis Protoplast Transient Expression System...... . . . 179 Se´verine Planchais, Laurent Camborde, and Isabelle Jupin 14 Relative Protein Lifetime Measurement in Plants Using Tandem Fluorescent Protein Timers...... . . . . 201 Hongtao Zhang, Eric Linster, Markus Wirtz, and Frederica L. Theodoulou 15 Detection and Quantification of Protein Aggregates in Plants.. . . 221 Ujjal Jyoti Phukan, Simon Stael, Amanda Gonc¸alves, Frank Van Breusegem, and Nu´ ria S. Coll 16 Using Intrinsic Fluorescence to Measure Protein Stability Upon Thermal and Chemical Denaturation..... . . . 229 Nathalia Vareja˜o and David Reverter PART V PROTEIN ISOLATION AND MASS SPECTROMETRIC ANALYSIS 17 Purification and Detection of Ubiquitinated Plant Proteins Using Tandem Ubiquitin Binding Entities.... . . . . . 245 DongHyuk Lee and Gitta Coaker 18 Titanium Oxide-Based Phosphopeptide Enrichment from Arabidopsis Seedlings....... . . . . 255 Sharon C. Mithoe and Frank L. H. Menke 19 Chloroplast Envelope Membrane Subfractionation from Arabidopsis and Pea...... . . . . . . 267 Annabel Dischinger and Serena Schwenkert 20 Chromatin Enrichment for Proteomics in Plants (ChEP-P).. . . . . 285 Isabel Cristina Ve´lez-Bermu´ dez and Wolfgang Schmidt 21 Proximity-Dependent In Vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha...... . . . 295 Katharina Melkonian, Sara Christina Stolze, Anne Harzen, and Hirofumi Nakagami 22 Tandem Mass Tag-Based Phosphoproteomics in Plants.... 309 Isabel Cristina Ve´lez-Bermu´ dez, Dharmesh Jain, Arya Ravindran, Chin-Wen Chen, Chuan-Chih Hsu, and Wolfgang Schmidt Contents xi PART VI STUDY OF PROTEASOME AND PROTEASES 23 Analysis of Peptide Hormone Maturation and Processing Specificity Using Isotope-Labeled Peptides...... 323 Stefanie Bru¨ck, Jens Pfannstiel, Gwyneth Ingram, Annick Stintzi, and Andreas Schaller 24 Improved Identification of Protease Cleavage Sites by In-gel Reductive Dimethylation........ . . 337 Stefanie Royek, Stefanie Bru¨ck, Jens Pfannstiel, Annick Stintzi, and Andreas Schaller 25 A Pipeline to Monitor Proteasome Homeostasis in Plants... . . . . . 351 Gautier Langin and Suayib U stu € ¨n PART VII BIOINFORMATIC ANALYSIS 26 Bioinformatic Tools for Exploring the SUMO Gene Network: An Update....... . . . 367 Pedro Humberto Castro, Miguel Aˆ ngelo Santos, Alexandre Papadopoulos Magalha˜es, Rui Manuel Tavares, and Herlander Azevedo 27 Coexpression Network Construction and Visualization from Transcriptomes Underlying ER Stress Responses...... . . . . . . 385 Dae Kwan Ko and Federica Brandizzi 28 Approaches for the Identification of Intrinsically Disordered Protein Domains........ . . . . . . 403 Huqiang Wang, Zhixiang Yang, and Dong Yang Index.......... 413 مشخصات فایل |
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| عنوان (Title): | Plant Proteostasis_ Methods and Protocols |
| نام فایل (File name): | 979-www.GeneProtocols.ir-Plant Proteostasis_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
پروتئوستاز گیاهی _ روش ها و پروتکل ها
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| ایجاد کننده: | L. Maria Lois, Marco Trujillo |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071627839 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 13.1 مگابایت |
| تعداد صفحات (Book length in pages): | 408 |
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