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فهرست مطالب: Preface . . . . . . . . . . . . . . vContributors. . . . . . . . . . . . . . . xi PART I BLOOD PROCESSING AND HANDLING STRATEGIES 1 Protocols for the Isolation of Platelets for Research and Contrast to Production of Platelet Concentrates for Transfusion . . . . . . . 3 2 Collection of Plasma Samples in Areas with Limited Healthcare Access . . . 19 3 Blood Collection Processing and Handling for Plasma and Serum Proteomics . . . . . . . . . 33 4 Preparation of Cryoprecipitate and Cryo-depleted Plasma for Proteomic Research Analysis . . . . . . . . . . 41 PART II DISCOVERY-BASED MASS SPECTROMETRY 5 High-Throughput Proteome Profiling of Plasma and Native Plasma Complexes Using Native Chromatography . . . . . . 53 6 High-Throughput and In-Depth Proteomic Profiling of 5 μL Plasma and Serum Using TMTpro 16-Plex . . . . . . . . . 81 7 An Optimized Data-Independent Acquisition Strategy for Comprehensive Analysis of Human Plasma Proteome . . . . . . . . 93 8 In-Depth Blood Proteome Profiling by Extensive Fractionation and Multiplexed Quantitative Mass Spectrometry . . . . . . . 109 9 Early Cancer Biomarker Discovery Using DIA-MS Proteomic Analysis of EVs from Peripheral Blood. . . . . . . 127 PART III DEVELOPMENTS IN TECHNOLOGIES FOR PLASMA PROTEOMICS 10 Strategies to Enrich, Identify, and Characterize Glycoproteome in Blood Plasma Using Liquid Chromatography High-Resolution Mass Spectrometry. . . . . . . . . 155 11 Proteome Analysis of Whole Blood Collected by Volumetric Absorptive Microsampling . . . . . . . . . . . . 173 12 Proteomic Applications and Considerations: From Research to Patient Care . . . . . . . . . . . . 181 PART IV ENRICHMENT & DETECTION STRATEGIES 13 Immunoaffinity Mass Spectrometry Diagnostic Tests for Multi-Biomarker Assays. . . . . . . . . . 195 14 Secretome Profile of Leukocyte-Platelet-Rich Fibrin (L-PRF) Membranes . . . . . . . . . . . . 207 15 Quantification of Proteins in Blood by Absorptive Microtiter Plate-Based Affinity Purification Coupled to Liquid Chromatography-Mass Spectrometry. . . . . . . . 221 16 Glycomics-Assisted Glycoproteomics Enables Deep and Unbiased N-Glycoproteome Profiling of Complex Biological Specimens . . . . . 235 17 The Small-Protein Enrichment Assay (SPEA) for Analysis of Low Abundance Peptide Hormones in Plasma . . . . . . . . . 265 PART V ANALYSIS OF EXTRACELLULAR VESICLES FROM BLOOD 18 In-Depth Proteomic Analysis of Blood Circulating Small Extracellular Vesicles . . . . . . . . . . . . 279 19 Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-Based Proteomic Identification . . . . . 291 20 Analysis of Extracellular Vesicle and Contaminant Markers in Blood Derivatives Using Multiple Reaction Monitoring . . . . . . 301 21 Generation of Red Blood Cell Nanovesicles as a Delivery Tool . . . . . 321 PART VI TARGETED-BASED MASS SPECTROMETRY 22 Progress in Targeted Mass Spectrometry (Parallel Accumulation-Serial Fragmentation) and Its Application in Plasma/Serum Proteomics . . 339 23 Offline Peptide Fractionation and Parallel Reaction Monitoring MS for the Quantitation of Low-Abundance Plasma Proteins . . . . . . 353 24 Profiling Serum Intact N-Glycopeptides Using Data-Independent Acquisition Mass Spectrometry . . . . . . . . 365 PART VII ASSAY DEVELOPMENT IN BIOMARKER DISCOVERY AND TRANSLATIONAL PROTEOMICS 25 Semi-Automated Lectin Magnetic Bead Array (LeMBA) for Translational Serum Glycoprotein Biomarker Discovery and Validation. . . 395 26 Accessing Antibody Reactivities in Serum or Plasma to (Auto-)antigens Using Multiplexed Bead-Based Protein Immunoassays . . . . . . . 413 27 Absolute Quantitative Targeted Proteomics Assays for Plasma Proteins. . . 439 PART VIII PLASMA-BASED PEPTIDE, LIPID, AND METABOLITE ASSAYS 28 Rapid and Quantitative Enrichment of Peptides from Plasma for Mass Spectrometric Analysis . . . . . . . 477 29 Comprehensive Targeted Lipidomic Profiling for Research and Clinical Applications . . . . . . . . . . . . 489 30 Multiplexed Bead-Based Peptide Immunoassays for the Detection of Antibody Reactivities . . . . . . . . . . 505 31 Array-Based Multiplex and High-Throughput Serology Assays . . . . . 535 PART IX SOFTWARE AND BIOINFORMATICS FOR THE PLASMA PROTEOME 32 Bioinformatics Tools and Knowledgebases to Assist Generating Targeted Assays for Plasma Proteomics . . . . . . 557 33 Use of Longitudinal Serum Analysis and Machine Learning to Develop a Classifier for Cancer Early Detection. . . . . . . . . 579 Index . . . . . . . . . . . . . . . 593 |
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فهرست به فارسی (ترجمه ماشینی): پیشگفتار . . . . . . . . . . . . . . v |
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مشخصات فایل |
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| عنوان (Title): | Serum_Plasma Proteomics_ Methods and Protocols |
| نام فایل (File name): | 1180-www.GeneProtocols.ir-Serum_Plasma Proteomics_ Methods and Protocols-Hu.pdf |
| عنوان فارسی (Title in Persian): |
پروتئومیکس سرم و پلاسما - روشها و پروتکل ها |
| ایجاد کننده: | David W. Greening, Richard J. Simpson |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071629772 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 14.9 مگابایت |
| تعداد صفحات (Book length in pages): | 591 |
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