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فهرست مطالب: Preface . . . . . . . . . . . . . . vContributors. . . . . . . . . . . . . . . xi PART I GLYCOSAMINOGLYCANS: STRATEGIES FOR DETECTION AND STRUCTURE CHARACTERIZATION 1 Detection of Glycosaminoglycans in Biological Specimens . . . . 3 Shaukat A. Khan, F. N. U. Nidhi, Paige C. Amendum, and Shunji Tomatsu 2 Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization of Chondroitin Sulfate Proteoglycans . . . . . . . 25 Tadahisa Mikami and Hiroshi Kitagawa 3 Proteoglycans: A Tool for Detecting Hyaluronan by ELISA-Like Methods. . . . . . . . . . . . 39 Daiana L. Vitale, Rosalı´a I. Cordo-Russo, and Laura Alaniz 4 Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix . . . . . . . . . 53 Arianna Parnigoni, Paola Moretto, Simona Rovera, Manuela Viola, Evgenia Karousou, Alberto Passi, and Davide Vigetti 5 Urinary Glycosaminoglycans: Characterization and Quantification . . 61 Naima Fdil and Es-Said Sabir 6 HPLC-MS Characterization of Tissue-Derived Heparan Sulfate and Chondroitin Sulfate . . . . . . . . . 71 Ga´ bor Toth and Lilla Turia´ k 7 Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues. . . . 91 Chrysostomi Gialeli and Isabel Gonc¸alves 8 Studying the Effects of Glycosaminoglycans in Cell Morphological Aspect with Scanning Electron Microscopy. . . . . . . . 99 Christos Koutsakis, Marco Franchi, Anastasia-Gerasimoula Tavianatou, Valentina Masola, and Nikos K. Karamanos PART II PROTEOGLYCANS: SEPARATION, ANALYSIS, AND INTERACTIONS 9 Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate Immunity . . . . . . . . . . . . . 109 Jinyang Zeng-Brouwers, Lisa Sophie Huber, Rosetta Merline, Jonel Trebicka, Malgorzata Wygrecka, and Liliana Schaefer 10 Analysis of Extracellular Vesicle-Associated Proteoglycans. . . . . 125 Juliana Poc¸as and Mattias Belting ixx Contents 11 Analysis of Aggrecan Glycanation by Western Blot in Cell Culture . . 141 Chiara Gramegna Tota, Alessandra Leone, Antonio Rossi, and Chiara Paganini 12 Repulsive Scaling Replica Exchange Molecular Dynamics in Modeling Protein-Glycosaminoglycan Complexes . . . . . . 153 Mateusz Marcisz, Martyna Maszota-Zieleniak, and Sergey A. Samsonov 13 In Vitro Investigation of Hyaluronan/CD44 Network . . . . . . . 169 Theodore Karalis, Paraskevi Heldin, and Spyros S. Skandalis 14 Separation and Identification of Native Proteoglycans by Composite Agarose-Polyacrylamide Gel Electrophoresis and Immunoblotting. . 187 James Melrose 15 Building, Visualizing, and Analyzing Glycosaminoglycan–Protein Interaction Networks. . . . . . . . . . . . 211 Sylvie Ricard-Blum PART III PROTEOGLYCANS RELATED MOLECULES (ENZYMES, INHIBITORS, AND EPIGENETICS) 16 An Enzymatic Activity Assay for Heparanase That Is Useful for Evaluating Clinically Relevant Inhibitors and Studying Kinetics. . . . . 227 Edward Hammond and Vito Ferro 17 A Method for Urinary Trypsin Inhibitor (UTI) Purification Combining Anion-Exchange Chromatography Enrichment and Preparative SDS-PAGE . . . . . . . . . . . 239 Gabriele Nieddu, Marilena Formato, and Antonio Junior Lepedda 18 Enzymatic Sequencing of Heparin Oligosaccharides Using Exolyase . . . . . 249 Qingdong Zhang, Danrong Lu, and Fuchuan Li 19 Evaluating the Effects of MicroRNAs on Proteoglycans and Matrix Constituents’ Expression and Functional Properties . . . 257 Zoi Piperigkou and Nikos K. Karamanos 20 Proteoglycan Expression Studied by MicroRNAs . . . . . . . 273 Nancy Adriana Espinoza-Sanchez, Fabian Troschel, Burkhard Greve, and Martin Go ¨tte 21 Modelling and Simulations of Extracellular Glycoproteins . . . . 293 Rajas M. Rao, Nicolas Belloy, Jean-Marc Crowet, Manuel Dauchez, and Ste´phanie Baud Index . . . . . . . . . . . . . . . 315 مشخصات فایل |
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| عنوان (Title): | Proteoglycans_ Methods and Protocols |
| نام فایل (File name): | 1003-www.GeneProtocols.ir-Proteoglycans_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
پروتئوگلایکان ها - روش ها و پروتکل ها
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| ایجاد کننده: | Nikos K. Karamanos |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071629451 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 10.9 مگابایت |
| تعداد صفحات (Book length in pages): | 321 |
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