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قابل دانلود از شنبه, ۲۳ ارديبهشت ۱۴۰۲
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فهرست مطالب: Preface . . . . . . . . . . . . . vContributors. . . . . . . . . ix Contents 1 Using FPbase: The Fluorescent Protein Database . . . . . . 1 Talley J. Lambert 2 Selecting the Fluorescent Protein for In Vivo Imaging Experiments . . . . 47 Mayank Sharma 3 Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo. . . . . . . . 53 Marcela Dı´az and Leonel Malacrida 4 Directed Evolution of Fluorescent Proteins in Bacteria . . . . . . . . 75 Sara Mattson, Geraldine N. Tran, and Erik A. Rodriguez 5 Fluorescent Proteins: Crystallization, Structural Determination, and Nonnatural Amino Acid Incorporation . . . . 99 Rochelle D. Ahmed, Husam Sabah Auhim, Harley L. Worthy, and D. Dafydd Jones 6 Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization. . . . . . . . . . 121 Alina Remeeva, Anna Yudenko, Vera V. Nazarenko, Oleg Semenov, Anastasia Smolentseva, Andrey Bogorodskiy, Ivan Maslov, Valentin Borshchevskiy, and Ivan Gushchin 7 Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. . . . . 143 Nora Lisa Bitzenhofer, Fabienne Hilgers, Gabriela N. Bosio, Joaquim Torra, Giorgia Casini, Felix R. M. Beinlich, Esther Knieps-Gru¨nhagen, Valentin Gordeliy, Karl-Erich Jaeger, Santi Nonell, Ulrich Krauss, Thomas Gensch, and Thomas Drepper 8 CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11 Tag into the Genome of a Human Cell Line . . . . . . . 185 Ryo Tamura and Daichi Kamiyama 9 Fluorescent Protein-Based Approaches for Subcellular Protein Localization in Plants . . . . . . . . . . . . . . 203 Mayank Sharma, Ralf Bernd Klo ¨sgen, and Bationa Bennewitz 10 Growth Rate Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions . . . . . . . 213 Kara L. Schneider, Lucas E. Reibenspies, Thomas Nystro ¨m, and Sviatlana Shashkova 11 DNA Visualization Using Fluorescent Proteins . . . . . . . . 223 Xuelin Jin, Y. Tehee Kim, and Kyubong Jo vii viii Contents 12 Quantification of Intracellular Citrate Concentrations with Genetically Encoded Biosensors. . . . . . . . . . . 247 Yufeng Zhao, Kei Takahashi-Yamashiro, Yi Shen, and Robert E. Campbell 13 Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out . . . . . . . . . . . . . 259 Finja Bohle, Andreas J. Meyer, and Stefanie J. Mueller-Schuessele 14 Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae. . . . . . 269 Mikel Lavilla-Puerta and Beatrice Giuntoli 15 Osmium-Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells. . . . . . . . . 287 Isei Tanida, Junji Yamaguchi, Soichiro Kakuta, and Yasuo Uchiyama 16 Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins. . . . . . . . . 299 Hebang Yao, Hongmin Cai, and Dianfan Li 17 Determining Protein-Protein Interaction with GFP-Trap Beads . . . . . . . 317 Irem Yilmazer, Melanie R. Abt, Yuanyuan Liang, David Seung, Samuel C. Zeeman, and Mayank Sharma Index . . . . . . . . . . . . . . 325 مشخصات فایل |
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| عنوان (Title): | Fluorescent Proteins_ Methods and Protocols |
| نام فایل (File name): | 964-www.GeneProtocols.ir-Fluorescent Proteins_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): |
پروتئین های فلورسنت - روشها و پروتکل ها
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| ایجاد کننده: | Mayank Sharma |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071626665 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 14.2 مگابایت |
| تعداد صفحات (Book length in pages): | 328 |
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