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قابل دانلود از سه شنبه, ۲۶ ارديبهشت ۱۴۰۲
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فهرست مطالب: Preface...................vContributors................... . xi 1 CCN Proteins (Cellular Communication Network Factors): Expanding Their Repertoire Toward a New Concept.........1 Masaharu Takigawa PART I BASIC METHODS AND PROTOCOLS 2 Optimal CCN4 Immunofluorescence for Tissue Microarray..... . . . 13 Pierre-Olivier Gaudreau, Isabelle Cousineau, and John Stagg 3 Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins.............. 23 Harumi Kawaki, Satoshi Kubota, and Masaharu Takigawa 4 Imaging of Molecular Interaction Between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes.......... . . . . 31 Mitsuhiro Hoshijima, Eriko Aoyama, Hiroshi Kamioka, and Masaharu Takigawa 5 Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-Free Fractions, and Cells......... . 39 Kisho Ono, Yuka Okusha, Manh Tien Tran, Koki Umemori, and Takanori Eguchi 6 Comprehensive Method for Exosome Isolation and Proteome Analysis for Detection of CCN Factors in/on Exosomes......59 Takanori Eguchi, Yuka Okusha, Yanyin Lu, Kisho Ono, Eman A. Taha, and Shiro Fukuoka 7 Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance.............. . . . . 77 Eriko Aoyama and Masaharu Takigawa 8 Protocols for Screening Peptides Binding to CCN Family Proteins and Their Extended Utility............. . . . . 87 Satoshi Kubota, Harumi Kawaki, and Masaharu Takigawa 9 Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism........... . 103 Takanori Eguchi, Yanyin Lu, Eman A. Taha, and Yuka Okusha 10 Analyses of the Posttranscriptional Regulation of CCN Genes: Approach to Multiple Steps of CCN2 Gene Expression........ . . . 127 Seiji Kondo, Satoshi Kubota, and Masaharu Takigawa vii viii Contents 11 Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication Network............. . . . . . 157 Yuka Okusha and Takanori Eguchi 12 Utilizing Public Molecular Biological Databases for CCN Family Research................ 169 Satoshi Kubota PART II FUNCTIONAL ANALYSIS 13 Evaluation of the Effects of CCN4 on Pancreatic Beta Cell Proliferation...............191 Rebeca Ferna´ndez-Ruiz and Rosa Gasa 14 Analysis of CCN Functions in Liver Regeneration After Partial Hepatectomy.............. . . . 209 Naiyuan Cheng, Ki-Hyun Kim, and Lester F. Lau 15 The Evaluation of Meniscus Regenerative Effects of LIPUS-Induced CCN Proteins: Induction by LIPUS of CCN2 and Meniscus-Related Genes in Cultured Meniscus Cells and Meniscus Tissues......223 Yusuke Kamatsuki, Eriko Aoyama, Takayuki Furumatsu, Toshifumi Ozaki, and Masaharu Takigawa 16 Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis................ . . . 237 Nobuo Takeshita and Teruko Takano-Yamamoto 17 Novel Cell Biological Assays for Measuring Bone Remodeling Activities of CCN Proteins............ . . . . . 255 Takashi Nishida, Satoshi Kubota, and Masaharu Takigawa 18 Imaging Analysis of Neurotrophic Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human-Induced Pluripotent Stem Cells.. . 269 Masaki Shoji and Takashi Kuzuhara 19 Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo.............. . 281 Kazumi Kawata and Satoshi Kubota PART III PATHOPHYSIOLOGY 20 Angiogenesis Assays for the Analysis of CCN Proteins....... . 295 Tsuyoshi Shimo, Mari Shimatani, Akihiko Tanimura, and Masaharu Takigawa 21 Using the Bleomycin-Induced Model of Fibrosis to Study the Contribution of CCN Proteins to Scleroderma Fibrosis.........309 Alexander Peidl, John Nguyen, Pratyusha Chitturi, Bruce L. Riser, and Andrew Leask 22 Atomic Force Microscopy-Based Measurements of Retinal Microvessel Stiffness in Mice with Endothelial-Specific Deletion of CCN1..... . 323 Brahim Chaqour, Maria B. Grant, Lester F. Lau, Biran Wang, Mateusz M. Urbanski, and Carmen V. Melendez-Vasquez Contents ix 23 Immunohistochemical Analysis of CCN2 in Experimental Fracture Healing Models................ 335 Tsuyoshi Shimo, Hiroaki Takebe, Saki Fujii, and Akihiro Hosoya 24 Mouse Models of Tumor Bone Metastasis and Invasion for Studying CCN Proteins................ . . 343 Tsuyoshi Shimo, Tatsuo Okui, Naohiro Horie, Kenji Yokozeki, Masaharu Takigawa, and Akira Sasaki 25 Regulation of CCN2 and Its Bioactivity by Advanced Glycation End Products.............. 355 Xiaoyu Wang, Danqing Min, and Stephen M. Twigg 26 Analysis of CCN4/WISP1 Effects on Joint Tissues Using Gain- and Loss-of-Function Approaches.......... 369 Martijn H. J. van den Bosch and Esmeralda N. Blaney Davidson 27 Generation of Conditional KO Mice of CCN2 and Its Function in the Kidney................. . . 391 Hideki Yokoi, Naohiro Toda, and Masashi Mukoyama 28 Analysis of the Function of CCN2 in Tubular Epithelium Cells with a Focus on Renal Fibrogenesis.......... . . . . 411 Hiroaki Amano, Tsutomu Inoue, Takeru Kusano, and Hirokazu Okada Index.................... 427 مشخصات فایل |
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| عنوان (Title): | CCN Proteins_ Methods and Protocols |
| نام فایل (File name): | 980-www.GeneProtocols.ir-CCN Proteins_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
پروتئین های CCN _ روش ها و پروتکل ها
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| ایجاد کننده: | Masaharu Takigawa |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071627433 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 17.1 مگابایت |
| تعداد صفحات (Book length in pages): | 424 |
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