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قابل دانلود از شنبه, ۳۰ ارديبهشت ۱۴۰۲
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فهرست مطالب: Preface............. . . . . vContributors............. . . . . ix 1 miRNA Biogenesis and Regulation of Diseases: An Updated Overview. . . . 1 Anchal Vishnoi and Sweta Rani 2 Exosomal MicroRNA Profiling........ . . . 13 Neda Rahimian, Javid Sadri Nahand, Michael R. Hamblin, and Hamed Mirzaei 3 MicroRNA Expression Profiling Using Agilent One-Color Microarray. . . . 49 Carmela Dell’Aversana, Giulia Sgueglia, Nunzio Del Gaudio, and Lucia Altucci 4 A Simple Method for Profiling and Analyzing MicroRNAs from Small Volume Samples Using a qPCR-Based Platform... . . . 65 Aisling Leavy and Eva M. Jimenez-Mateos 5 Exosomal MicroRNAs: Comprehensive Methods from Exosome Isolation to miRNA Extraction and Purity Analysis...... 75 D’Agostino Erika, Muro Annamaria, Sgueglia Giulia, Massaro Crescenzo, Dell’Aversana Carmela, and Altucci Lucia 6 Detection of MicroRNAs in Brain Slices Using In Situ Hybridization. . . . . 93 Sean Quinlan, Christine Henke, Gary P. Brennan, David C. Henshall, and Eva M. Jimenez-Mateos 7 MicroRNA Profiling of Cell Lines and Xenografts by Quantitative PCR: MicroRNA Expression Level Determination by qPCR... . . 101 Ariadna Boloix and Miguel F. Segura 8 Assessment of Basic Biological Functions Exerted by miRNAs.... 115 Ellen King, John Nolan, and Olga Piskareva 9 Serum MicroRNAs Profiling in Age-Related Macular Degeneration... 123 Hanan Elshelmani, David Keegan, and Sweta Rani 10 Exosomal MicroRNA Discovery in Age-Related Macular Degeneration. . . 137 Hanan Elshelmani and Sweta Rani 11 MicroRNA Profiling Using a PCR-Based Method..... . 159 Giuliana A. de Ferronato, Marcela B. Cerezetti, Alessandra Bridi, Cibele M. Prado, Gislaine dos Santos, Nata´lia M. Bastos, Paola M. S. da Rosa, Juliana G. Ferst, and Juliano C. da Silveira 12 Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line.......... . . . . 171 Manoela Marques Ortega and Hakim Bouamar 13 Evaluating Single-Nucleotide Variants in MicroRNA Targeting Sites and Mature MicroRNA In Vitro Cell Culture by Luciferase Reporter Gene Assays.......... . 185 Je´ssica Silva dos Santos, Gabriel Alves Bonafe´, Gustavo Jacob Lourenc¸o, and Manoela Marques Ortega vii viii Contents 14 Assessment of Cell Cytotoxicity in 3D Biomaterial Scaffolds Following miRNA Transfection......... . . 203 Elizabeth Sainsbury, Lara Costard, Fergal J. O’Brien, and Caroline M. Curtin 15 Evaluation of miRNA Expression in 3D In Vitro Scaffold-Based Cancer Models........... . . 211 Catherine Murphy, Ciara Gallagher, and Olga Piskareva 16 Bioinformatics Analysis of miRNA Sequencing Data.... . . . . 225 Hrishikesh A. Lokhande 17 Plant MicroRNA Identification and Annotation Using Deep Sequencing Data............ 239 Zheng Kuang, Yongxin Zhao, and Xiaozeng Yang Index.............. 251 مشخصات فایل |
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| عنوان (Title): | MicroRNA Profiling_ Methods and Protocols |
| نام فایل (File name): | 987-www.GeneProtocols.ir-MicroRNA Profiling_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
پروفایل میکرو آر ان ای - روشها و پروتکل ها
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| ایجاد کننده: | Sweta Rani |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071628225 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 11.4 مگابایت |
| تعداد صفحات (Book length in pages): | 258 |
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