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فهرست مطالب: Preface . . . . . . . . . . . . . . . . . vContributors. . . . . . . . . . . . . xi PART I DETECTION AND QUANTIFICATION OF PADPR 1 Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High-Performance Liquid Chromatography, and Capillary Electrophoresis. . . . . . . . . 3 Mohsen Badiee, Audrey Boutonnet, Dat Phan, and Anthony K. L. Leung 2 Detecting and Quantifying pADPr In Vivo. . . . . . . . . 23 Andrew M. Lamade, Yaming Chen, Carla J. Johnson, Hu¨lya Bayır, and Robert S. B. Clark 3 Quantitative Analysis of Nuclear Poly(ADP-Ribose) Dynamics in Response to Laser-Induced DNA Damage. . . . . . . 43 Christopher A. Koczor, Kate M. Saville, Rasha Q. Al-Rahahleh, Joel F. Andrews, Jianfeng Li, and Robert W. Sobol 4 Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly (ADP-Ribose). . . . . . . . 61 Johannes Rudolph and Karolin Luger 5 Detecting Poly (ADP-Ribose) In Vitro and in Cells Using PAR Trackers . . . . . . . . . . . . . . . 75 Sridevi Challa, Amy L. Whitaker, and W. Lee Kraus 6 An Enzyme-Linked Immunosorbent Assay to Quantify Poly (ADP-Ribose) Level In Vivo . . . . . . . . . 91 Chieri Ida, Sachiko Yamashita, Takayuki Eguchi, Yasuhito Kuroda, Setsu Nakae, Yoshisuke Nishi, Kazuo Kamemura, Tsuyoshi Shirai, Tamio Mizukami, Masakazu Tanaka, Joel Moss, and Masanao Miwa 7 Subcellular Quantitation of ADP-Ribosylation by High-Content Microscopy . . . . . . . . . . . 101 Lukas Muskalla, Anka Gu¨ldenpfennig, and Michael O. Hottiger 8 A Simple Method to Study ADP-Ribosylation Reversal: From Function to Drug Discovery . . . . . . . . 111 Johannes Gregor Matthias Rack and Ivan Ahel PART II IDENTIFICATION OF PROTEIN TARGETS 9 Immunoprecipitation Using Mono-ADP-Ribosylation-Specific Antibodies . . . . . . . . . . . . . 135 Helen Dauben and Ivan Matic 10 A Clickable NAD+ Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins. . . . 147 Dongsheng Yao, Heba Ahmed, and Junqi Song viiviii Contents 11 Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells . . . 157 Dan Huang, Andrea D. Edwards, Xuan Gong, and W. Lee Kraus PART III FUNCTIONAL ANALYSIS 12 Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer. . . . . . . . . . . 195 Nura Lutfi, Carlos Martı´nez, and Jose´ Ye´lamos 13 Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2): A Cation Channel Activated by ADP-Ribose and Involved in Cell Death . . . . . . . . . . 213 Hannah K. Hall and David W. Koh 14 Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function . . . . . . . . . . . . . 227 Tu¨nde Kova´cs, Bogla´rka Rauch, Edit Miko, and Pe´ter Bai 15 Characterizing ADP-Ribosylation Sites Using Af1521 Enrichment Coupled to ETD-Based Mass Spectrometry . . . . . . . . 251 Holda A. Anagho, Jonas D. Elsborg, Ivo A. Hendriks, Sara C. Buch-Larsen, and Michael L. Nielsen 16 Cytological Approaches to Visualize Intracellular Dynamics of RNA-Binding Proteins at Active Genes in Drosophila. . . . . . . . 271 Anand K. Singh and Subhash C. Lakhotia PART IV POLY(ADP-RIBOSYL)ATING PATHWAY IN CHROMATIN AND GENE EXPRESSION 17 Chromatin Immunoprecipitation Approach to Determine How PARP1 Domains Affect Binding Pattern to Chromatin . . . . . 297 Guillaume Bordet, Gbolahan Bamgbose, Sayem H. Bhuiyam, Sarah Johnson, and Alexei V. Tulin 18 Approach to Measuring the Effect of PARP1 on RNA Polymerase II Elongation Rates . . . . . . . . . . . 315 Hejer Dhahri, Elena Matveeva, and Yvonne Fondufe-Mittendorf 19 Examining the Effect of PARP-1 Inhibitors on Transcriptional Activity of Androgen Receptor in Prostate Cancer Cells. . . . . . . . . 329 Peter Makhov, Rushaniya Fazliyeva, Antonio Tufano, Robert G. Uzzo, and Vladimir M. Kolenko PART V USING ANIMAL MODELS 20 Using Drosophila Genetics to Identify Factors that Affect PARP1 Activity In Vivo. . . . . . . . . . . . . 339 Guillaume Bordet and Alexei V. Tulin 21 Generating PARP Knockout D. melanogaster with CRISPR/Cas9 System . 353 Yaroslava Karpova and Alexei V. Tulin 22 TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice. . . . 363 Yaroslava Karpova and Alexei V. TulinContents ix PART VI DESIGNING AND TESTING PARP-1 INHIBITORS 23 Cell-Based Screening for New PARP Inhibitors Utilizing PARG-Mutated Mouse Embryonic Stem Cells . . . . . . . . . 375 Yaroslava Karpova, Danping Guo, and Alexei V. Tulin 24 Quantification of PARP7 Protein Levels and PARP7 Inhibitor Target Engagement in Cells Using a Split Nanoluciferase System . . . . . 387 Anna K. Duell, Daniel J. Sanderson, and Michael S. Cohen PART VII TARGETING THE UNDERSTUDIED COMPONENTS OF PADPR PATHWAY 25 Purification of Recombinant Human PARG and Activity Assays . . . . . . 399 Jean-Christophe Ame´ and Franc¸oise Dantzer 26 Purification of Recombinant Human PARP-3 . . . . . . 419 Jean-Christophe Ame´, Leonel Nguekeu-Zebase, Daisy Harwood, Zuleyha Yildirim, Lisa Roegel, Agathe Boos, and Franc¸oise Dantzer Index . . . . . . . . . . . . . . 443 مشخصات فایل |
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| عنوان (Title): | Poly(ADP-Ribose) Polymerase_ Methods and Protocols |
| نام فایل (File name): | 998-www.GeneProtocols.ir-Poly(ADP-Ribose) Polymerase_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
پلی ADP ریبوز پلیمراز - روش ها و پروتکل ها
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| ایجاد کننده: | Alexei V. Tulin |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071628904 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 15.3 مگابایت |
| تعداد صفحات (Book length in pages): | 441 |
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