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فهرست مطالب: Preface . . . . vContributors. . . . . ix PART I METHODS TO STUDY THE NATURE AND FUNCTION OF PIRNAS IN NON-CLASSICAL ORGANISMS 1 Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides . . . 3 Rebecca Halbach and Pascal Miesen 2 CRISPR-Mediated Genome Engineering in Aedes aegypti. . . . . 23 Ruichen Sun, Ming Li, Conor J. McMeniman, and Omar S. Akbari 3 PIWI-Directed DNA Elimination for Tetrahymena Genetics . . 53 Salman Shehzada and Kazufumi Mochizuki 4 Planarian PIWI–piRNA Interaction Analysis Using Immunoprecipitation and piRNA Sequencing . . . . . 69 Makoto Kashima, Atsumi Miyata, and Norito Shibata 5 Isolation and Processing of Bovine Oocytes for Small RNA Sequencing . . 83 Minjie Tan, Helena T. A. van Tol, and Elke F. Roovers 6 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther . . . . . 93 Koji Koizumi and Reina Komiya PART II METHODS TO STUDY ROLES OF PIRNAS IN CLASSIC MODEL ORGANISMS 7 Cloning, Sequencing, and Linkage Analysis of piRNAs . . 107 Rippei Hayashi 8 Drosophila Genetic Resources for Elucidating piRNA Pathway . . . . . 135 Kuniaki Saito 9 Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System . . 143 Chikara Takeuchi, Kensaku Murano, Mitsuru Ishikawa, Hideyuki Okano, and Yuka W. Iwasaki PART III METHODS TO STUDY NUCLEAR REGULATION BY OTHER NON-CODING RNAS 10 Whole-Mount RNA FISH Combined with Immunofluorescence for the Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline. . 157 Valeriya Morgunova, Maria M. Sukhova, and Alla Kalmykova vii11 CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells . . . 171 Akihiko Sakashita, Masaru Ariura, and Satoshi H. Namekawa 12 Method for Evaluating Effects of Non-coding RNAs on Nucleosome Stability . . . . 195 Mariko Dacher, Risa Fujita, Tomoya Kujirai, and Hitoshi Kurumizaka 13 Revisiting the Glass Treatment for Single-Molecule Analysis of ncRNA Function . . . 209 Shuting Shen, Masahiro Naganuma, Yukihide Tomari, and Hisashi Tadakuma 14 Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification. . 233 Wan Kin Au Yeung and Hiroyuki Sasaki 15 Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seq. . 251 Abd-El Monsif Shawky, Mahmoud Dondeti, Zissimos Mourelatos, and Anastasios Vourekas 16 UPA-Seq-Based Search Method for Functional lncRNA Candidates. 269 Saori Yokoi and Shinichi Nakagawa 17 Large-Scale Analysis of RNA–Protein Interactions for Functional RNA Motif Discovery Using FOREST . 279 Emi Miyashita, Kaoru R. Komatsu, and Hirohide Saito PART IV BIOINFORMATIC AND BIOPHYSICAL METHODS TO STUDY NON-CODING RNAS 18 Computational Methods for the Discovery and Annotation of Viral Integrations. . . 293 Umberto Palatini, Elisa Pischedda, and Mariangela Bonizzoni 19 Bioinformatics Approaches for Determining the Functional Impact of Repetitive Elements on Non-coding RNAs . . . . . 315 Chao Zeng, Atsushi Takeda, Kotaro Sekine, Naoki Osato, Tsukasa Fukunaga, and Michiaki Hamada 20 Extending and Running the Mosquito Small RNA Genomics Resource Pipeline . . . . . 341 Gargi Dayama, Katia Bulekova, and Nelson C. Lau 21 Preparation of Non-overlapping Transposable Elements (TEs) Annotation by Interval Tree. . . . . . 353 Shohei Kojima 22 Statistical Thermodynamics Approach for Intracellular Phase Separation. . . . . . 361 Tomohiro Yamazaki and Tetsuya Yamamoto Index . . . . . 395 مشخصات فایل |
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| عنوان (Title): | piRNA_ Methods and Protocols |
| نام فایل (File name): | 923-www.GeneProtocols.ir-piRNA_ Methods and Protocols-Humana Press (2022).pdf |
| عنوان فارسی (Title in Persian): | پی آی آر ان ای- روشها و پروتکل ها |
| ایجاد کننده: | Nicholas F. Parrish, Yuka W. Iwasaki |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 9781071623794, 9781071623800 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 11.3 مگابایت |
| تعداد صفحات (Book length in pages): | 396 |
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