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فهرست مطالب: Preface .......... . . . . . vContributors.......... . xi PART I INTRODUCTION 1 Two-Dimensional Gel Electrophoresis and 2D-DIGE .... . 3 Paula Meleady PART II DIFFERENCE GEL ELECTROPHORESIS 2 Top-Down Proteomics and Comparative 2D-DIGE Analysis ... . . 19 Kay Ohlendieck 3 DIGE Analysis Software and Protein Identification Approaches... 39 Paul Dowling PART III DIGE APPLICATIONS 4 Native DIGE for Quantitative and Functional Analysis of Protein Interactomes ......... . . 53 Diksha Dani and Norbert A. Dencher 5 Comparative Two-Dimensional Fluorescence Gel Electrophoresis .. . . . . 71 Doreen Ackermann and Simone Ko ¨nig 6 Identification of Ubiquitination-Associated Proteins Using 2D-DIGE.. . 83 Paul Dowling and Despina Bazou 7 DIGE-Based Phosphoproteomic Analysis ..... . . . . . 97 Taras Stasyk and Lukas Alfons Huber 8 DIGE-Based Biomarker Discovery in Blood Cancers .... . . 105 Katie Dunphy and Paul Dowling 9 DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue...113 Paul Dowling 10 DIGE Analysis of ProteoMiner™ Fractionated Serum/Plasma Samples .119 Sandra Murphy and Paul Dowling 11 Comparative 3-Sample 2D-DIGE Analysis of Skeletal Muscles ... 127 Kay Ohlendieck 12 Proteomic Identification of Saliva Proteins as Noninvasive Diagnostic Biomarkers ........ 147 Eleanor M. O’Sullivan, Paul Dowling, Dieter Swandulla, and Kay Ohlendieck 13 Analysis of the Saliva Proteome Using 2D-DIGE .... . . . . . 169 Paul Dowling and Eleanor M. O’Sullivan vii viii Contents PART IV COMPARATIVE DIGE TISSUE PROTEOMICS 14 DIGE Analysis of Clinical Specimens ...... . . 177 Cecilia Gelfi and Daniele Capitanio 15 DIGE Analysis of Animal Tissues ...... . . . . . 201 Alessio Di Luca, Ruth Hamill, Anne Maria Mullen, and Giuliano Elia 16 2D-DIGE Proteomic Analysis of Mouse Liver Within 1 Week... . 217 Shotaro Kamata and Isao Ishii 17 2D-DIGE Analysis of Liver Disease in Mice..... . . . 231 Aı ¨sha Callebaut, Rita Derua, Lut Overbergh, and Veerle Janssens 18 Two-Dye Versus Three-Dye DIGE for Comparative Testis Tissue Proteomic Analysis....... . . . . . 245 Ashling Holland 19 Two-CyDye-Based 2D-DIGE Analysis of Aged Human Muscle Biopsy Specimens ........ . . . . . 265 Kay Ohlendieck 20 Identification of Subproteomic Markers for Skeletal Muscle Profiling ........291 Paul Dowling, Stephen Gargan, Dieter Swandulla, and Kay Ohlendieck 21 DIGE Analysis of Fish Tissues ....... . 303 Joanna Nynca, Mariola A. Dietrich, and Andrzej Ciereszko PART V AUXILIARY DIGE METHODS 22 Sample Preparation and Protein Determination for 2D-DIGE Proteomics....... . . . . . 325 Stephen Gargan and Kay Ohlendieck 23 Protein Digestion for 2D-DIGE Analysis ..... . . . . . 339 Sandra Murphy and Kay Ohlendieck 24 Subcellular Fractionation for DIGE-Based Proteomics .... 351 Sandra Murphy 25 DIGE Analysis of Immunodepleted Plasma ..... . . . 363 Paul Dowling and Kay Ohlendieck 26 Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue....... . . . . 377 Sandra Murphy, Margit Zweyer, Dieter Swandulla, and Kay Ohlendieck PART VI DIGE VERIFICATION ANALYSIS 27 Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments Using Enzyme Assays ... . . . 399 Andrew Dowd Contents ix 28 Enzyme Assay Methods to Validate DIGE Proteomics Data... . . . 421 Andrew Dowd 29 Immunoblot Analysis of DIGE-Based Proteomics .... . . . . 429 Martin Landsberger and Heinrich Brinkmeier 30 Verification of Protein Changes Determined by 2D-DIGE Based Proteomics Using Immunofluorescence Microscopy .... . . 445 Margit Zweyer, Kay Ohlendieck, and Dieter Swandulla 31 Histological and Histochemical Microscopy Used to Verify 2D-DIGE Pathoproteomics....... . . . 465 Margit Zweyer, Kay Ohlendieck, and Dieter Swandulla Index ..........481 مشخصات فایل |
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| عنوان (Title): | Difference Gel Electrophoresis_ Methods and Protocols |
| نام فایل (File name): | 988-www.GeneProtocols.ir-Difference Gel Electrophoresis_ Methods and Protocols-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
ژل الکتروفورز افتراقی - روشها و پروتکل ها
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| ایجاد کننده: | Kay Ohlendieck |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071628300 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 15.2 مگابایت |
| تعداد صفحات (Book length in pages): | 477 |
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