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فهرست مطالب: Preface . . . . . . . . . . . . . . . vContributors. . . . . . . . . . . xi PART I CHAIN DIVERSITY 1 Thioester and Oxyester Linkages in the Ubiquitin System. . . . . . . . 3 Alba Ferri-Blazquez, Ernst Jarosch, and Thomas Sommer 2 Getting to the Root of Branched Ubiquitin Chains: A Review of Current Methods and Functions . . . . . . . . . 19 Anita Waltho and Thomas Sommer PART II UB AND UBL CHEMICAL TOOLS 3 Chemical Synthesis of Non-hydrolyzable Ubiquitin (-Like) Hybrid Chains . . . . . . . . . 41 David A. Pe´rez Berrocal, Gerbrand J. van der Heden van Noort, and Monique P. C. Mulder 4 Total Linear Chemical Synthesis of LC3A and LC3B . . . . . . 51 Yara Huppelschoten, Jens Buchardt, Thomas Eiland Nielsen, and Gerbrand J. van der Heden van Noort PART III METHODS TO STUDY UBIQUITIN CONJUGATING ENZYMES (E3S) 5 A Microbead-Based Flow Cytometry Assay to Assess the Activity of Ubiquitin and Ubiquitin-Like Conjugating Enzymes . . . 65 Clara Recasens-Zorzo, Pierre Gaˆtel, Fre´de´rique Brockly, and Guillaume Bossis 6 Monitoring HECT Ubiquitination Activity In Vitro . . . . . . 81 Vincenzo Taibi, Simona Polo, and Elena Maspero PART IV METHODS TO STUDY DUBS 7 RNAi-Based Screening for the Identification of Specific Substrate-Deubiquitinase Pairs . . . . . . . 95 Nagore Elu, Natalia Presa, and Ugo Mayor 8 Strategies for Monitoring “Ubiquitin C-Terminal Hydrolase 1” (Yuh1) Activity . . . . . . . . . 107 Shahaf Saad, Eden Berda, Yuval Klein, Suha Issa, and Elah Pick ix x Contents PART V METHODS TO IDENTIFY NEW TARGETS AND REGULATORS 9 Isolation and Mass Spectrometry Identification of K48 and K63 Ubiquitin Proteome Using Chain-Specific Nanobodies . . . . 125 Maria Gonzalez-Santamarta, Laurie Ceccato, Ana Sofia Carvalho, Jean-Christophe Rain, Rune Matthiesen, and Manuel S. Rodriguez 10 A Mass Spectrometry-Based Strategy for Mapping Modification Sites for the Ubiquitin-Like Modifier NEDD8 . . . . . . . . . . 137 Caio A. B. Oliveira, Ekaterina Isaakova, Petra Beli, and Dimitris P. Xirodimas 11 Isolation of the Hepatic Ubiquitome/NEDDylome by Streptavidin Pull-Down Assay in the Biotinylated Ubiquitin (bioUb)/Biotinylated NEDD8 (bioNEDD8) Transgenic Mice. . . . . . 151 Marina Serrano-Macia´, Teresa Cardoso Delgado, and Marı´a Luz Martı´nez-Chantar 12 The Lambda Display Technology: A Useful Tool for the Identification of Ubiquitin-and Ubiquitin-Like-Binding Domains . . . . . . . 163 Elena Santonico 13 SUMO-ID: A Strategy for the Identification of SUMO-Dependent Proximal Interactors . . . . . . . . . . 177 Orhi Barroso-Gomila, Ugo Mayor, Rosa Barrio, and James D. Sutherland 14 Analysis of ATG8 Family Members Using LC3-Interacting Regions (LIR)-Based Molecular Traps. . . . . . . 191 Gre´goire Quinet, Pierre Ge´nin, Naima Belgareh-Touze´, Oznur Ozturk, Robert Weil, Mickael M. Cohen, Renaud Legouis, and Manuel S. Rodriguez 15 A Computational Tool for Analysis of Mass Spectrometry Data of Ubiquitin-Enriched Samples . . . . . . . 205 Rune Matthiesen, Manuel S. Rodriguez, and Ana Sofia Carvalho PART VI METHODS TO MEASURE PROTEASOME ACTIVITY 16 In-Plate and In-Gel Assays for the Assessment of Proteasome Activity in Caenorhabditis elegans . . . . . . . . . . . . 217 Eleni Panagiotidou, Anna Gioran, and Niki Chondrogianni 17 Isolation of Proteasome-Trapped Peptides (PTPs) for Degradome Analysis . . . . . . . 229 Indrajit Sahu, Manisha Priyadarsini Sahoo, Oded Kleifeld, and Michael H. Glickman Index . . . . . . . . . . 243 مشخصات فایل |
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| عنوان (Title): | The Ubiquitin Code |
| نام فایل (File name): | 993-www.GeneProtocols.ir-The Ubiquitin Code-Humana Press (2023).pdf |
| عنوان فارسی (Title in Persian): |
کد یوبیکوتین
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| ایجاد کننده: | Manuel S. Rodriguez, Rosa Barrio |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2023 |
| شابک ISBN: | 9781071628584 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 8.04 مگابایت |
| تعداد صفحات (Book length in pages): | 245 |
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