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فهرست مطالب: Preface . . . . . . . . . . . . . . . vContributors. . . . . . . . . . . xi PART I THE CANDIDA GENOME 1 CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans . . . . . . . 3 Ben A. Evans and Douglas A. Bernstein 2 Plasmid-Based CRISPR–Cas9 Editing in Multiple Candida Species . . 13 Lisa Lombardi and Geraldine Butler 3 A Proteomic Approach for the Quantification of Posttranslational Protein Lysine Acetylation in Candida albicans. . . . . . . . . . . 41 Raju Shivarathri, Manju Chauhan, Rounik Mazumdar, Phan Canh Trinh, Wolfgang Reiter, Markus Hartl, Karl Kuchler, and Neeraj Chauhan 4 How to Use the Candida Genome Database . . . . 55 Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock 5 Subtelomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata . . . . . . . 71 Eunice Lopez-Fuentes, Grecia Herna´ndez-Herna´ndez, Alejandro De Las Pen˜as, and Irene Castan˜o 6 Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans . . . . . . . . 91 Virginia Glazer and Damian Krysan PART II ANTIFUNGAL DISCOVERY AND RESISTANCE 7 Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance . . . . . . . . . . . 103 Jane Usher 8 Identifying Specific Small Molecule–Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS). . . . . . . . . . 115 Jessica Regan and Glen E. Palmer 9 High-Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis . . . . . . 127 Mohit Kumar, Rajendra Prasad, and Ashutosh Singh 10 Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous Saccharomyces cerevisiae Mutant Library. . . . . . . . . . 141 Lulu Zhang, Dongmei Li, Richard Calderone, and Xiaodong She vii viii Contents 11 Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans . . . . . . . 151 Dongmei Li, Xiaodong She, and Richard Calderone PART III PATHOGENESIS AND VIRULENCE 12 In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin . . . . . . . . . . . . . 163 Sejeong Lee, Nessim Kichik, Olivia W. Hepworth, Jonathan P. Richardson, and Julian R. Naglik 13 Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors. . . . . . . . . . . 177 Liesbeth Demuyser, Wouter Van Genechten, and Patrick Van Dijck 14 Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy . . . . . . . . . . . 193 Junko Yano and Paul L. Fidel Jr 15 A Simple Method for Growth of Candida albicans Biofilms Under Continuous Media Flow and for Recovery of Biofilm Dispersed Cells. . . 219 Priya Uppuluri 16 A Simple 96-Well Plate-Based Method for Development of Candida Biofilms Under Static Conditions . . . 225 Priya Uppuluri 17 Comparative Analysis of the Fitness of Candida albicans Strains During Colonization of the Mice Gastrointestinal Tract . . . 233 Daniel Prieto and Jesu´ s Pla 18 Detection and Identification of Candida auris from Clinical Skin Swabs. . . . . . . 245 Milena Kordalewska and David S. Perlin 19 Production and Isolation of the Candida Species Biofilm Extracellular Matrix . . . . . . . . 257 Marienella Hereidia and David Andes PART IV COMMUNITIES 20 Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model . 271 Jesu´ s A. Romo and Carol A. Kumamoto 21 Analysis of Gut Microbiome Using Fecal Samples. . . . . . . . . 287 Ahmed Gamal, Mayyadah Alabdely, Kelly Steller, Hilmi Al-Shakhir, Mohammed Elshaer, and Mahmoud Ghannoum 22 Measurements of Treg Cell Induction by Candida albicans DNA Using Flow Cytometry. . . . . . . . . 301 Dongmei Li, Jie Cheng, Richard Calderone, and Joseph A. Bellanti Contents ix PART V IMMUNITY 23 Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure . . . . 309 Ebrima Bojang, Rebecca A. Drummond, and Rebecca A. Hall 24 Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans . . . . . . . 323 Michael D. Kruppa, Douglas W. Lowman, Harry E. Ensley, Zuchao Ma, Bridget Graves, Jennifer Kintner, Jennifer V. Hall, Tammy R. Ozment, and David L. Williams 25 Understanding the Protective Role of IL-17 During Oropharyngeal Candidiasis. . . . . . . . . . . 361 Jessica Saul-McBeth and Heather R. Conti Index . . . . . . . 375 مشخصات فایل |
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| عنوان (Title): | Candida Species_ Methods and Protocols |
| نام فایل (File name): | 941-www.GeneProtocols.ir-Candida Species_ Methods and Protocols-Humana (2022).pdf |
| عنوان فارسی (Title in Persian): | گونه کاندیدا- روشها و پروتکل ها |
| ایجاد کننده: | Richard Calderone |
| زبان (Language): | انگلیسی English |
| سال انتشار: | 2022 |
| شابک ISBN: | 1071625489, 9781071625484 |
| نوع سند (Doc. type): | کتاب |
| فرمت (File extention): | |
| حجم فایل (File size): | 11.4 مگابایت |
| تعداد صفحات (Book length in pages): | 388 |
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